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Bacillus, bacterial agent, preparation method and applications thereof

A technology of bacillus and inoculum, which is applied in the field of microorganisms to achieve the effects of strong environmental adaptability and environmental friendliness, significant poisoning effect and low cost

Active Publication Date: 2015-03-11
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the available data, the preparation of Bacillus amyloliquefaciens and its preparation derived from plant endogenous and the research of using the preparation to prevent and control plant root-knot nematode have never been reported.

Method used

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  • Bacillus, bacterial agent, preparation method and applications thereof

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preparation example Construction

[0042] The preparation method of the bacterial agent with bacillus as the active ingredient of the present invention comprises the steps of inclined plane culture, seed cultivation, fermentation, liquid bacterial agent preparation, and solid bacterial agent preparation steps, specifically comprising:

[0043] A, slant culture: Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) WY11 was inoculated on the slant medium, and cultured at 28-30°C for 48 hours to obtain slant strains;

[0044] B. Seed culture: inoculate the slant strains into the liquid medium, and cultivate them at a temperature of 28-34°C for 18-24 hours to obtain liquid-fermented seeds;

[0045] C. Fermentation: inoculate the liquid fermented seeds into the fermenter according to the amount of 3~5% of the medium volume, cultivate at 28~34°C for 36~48h, the stirring speed is 120~150rpm, and the ventilation rate is 5~10%, and the solution is obtained. Bacillus amylogenus WY11 fermentation broth;

[0046] D. ...

Embodiment 1

[0064] ——Preparation of preparations with Bacillus amyloliquefaciens WY11 as the active ingredient

[0065] A, slant culture: Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) WY11 was inoculated on the agar medium and cultured at 30°C for 48 hours to obtain slant strains.

[0066] B. Seed culture: the above-mentioned slant strains were inserted into the seed culture solution, cultured at 30° C. for 24 hours, and the rotation speed of the shaker flask was 150 rpm to obtain liquid fermented seeds. Ingredients of seed culture solution: 3% beef extract, 0.5% peptone, 0.5% sodium chloride, the rest is water, and the pH value is 7.

[0067] C. Liquid fermentation culture: according to the inoculum amount of 5%, inoculate the above-mentioned liquid fermentation seeds into a fermenter equipped with a liquid fermentation system, and cultivate them at 32°C for 48 hours, with a stirring speed of 150rpm and an air flow of 8%, to obtain starch decomposing Bacillus WY11 fermenta...

Embodiment 2

[0070] ——Preparation of preparations with Bacillus amyloliquefaciens WY11 as the active ingredient

[0071] It is basically the same as Example 1, except that: A, the temperature of slant culture is 28°C. B. The temperature of the seed culture is 28° C., the time is 18 hours, and the rotation speed of the shaker flask is 120 rpm. The pH value of the seed culture solution was 6.5. C. The liquid fermentation culture is inoculated at 3%, the temperature is 28°C, the time is 36h, the stirring speed is 120rpm, and the ventilation rate is 5%. The components of the fermentation medium are: 1% bean cake powder, 1% soluble starch, 0.5% beef extract, NaCNO 3 0.02%, CaCO 3 0.1%.

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Abstract

The present invention discloses a strain of bacillus, wherein the bacillus is Bacillusamyloliquefaciens WY11, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 11, 2012, and a preservation number is CGMCC No.6663. The bacterial agent adopting the bacillus as an active component is prepared by carrying out slant culture, seed culture and fermentation on the Bacillusamyloliquefaciens WY11 and adding an active agent. The preparation method for the bacterial agent adopting the bacillus as the active component comprises steps such as slant culture, seed culture, fermentation, liquid bacterial agent preparation and solid bacterial agent preparation. The invention further provides applications of the bacterial agent adopting the bacillus as the active component in plant root-knot nematode homicidal poisoning drug preparation. According to the present invention, the bacillus and the bacterial agent adopting the bacillus as the active component provide significant homicidal poisoning effects for plant root-knot nematodes; use is convenient; no toxic and harmful effect is generated to humans and animals; and plant microbe ecology flora can be regulated, and plant growth can be promoted.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus and its bacterial agent, preparation method and application. Background technique [0002] Plant-parasitic nematodes are one of the major pathogens of plants, causing an estimated $100 billion in annual losses. It is widely distributed all over the world and has a wide range of hosts. It can make it easier for bacteria and fungi to infect plants, and is one of the important reasons for inducing plant diseases. Tobacco root-knot nematode is two diseases that seriously harm tobacco. With the development of tobacco production and the increase of continuous cropping area, the damage is becoming more and more serious. Nematode disease is one of the main limiting factors of tobacco production in many countries. The annual incidence of root-knot nematode disease in Yunnan Province alone is 20,000 to 30,000 hectares, and the diseased plants cause a 30%-50% ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/07
Inventor 夏振远汪安云吴玉萍莫笑晗雷丽萍
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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