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Method for inoculating tomatoes with tomato yellow leaf curl viruses

A technology of tomato yellowing, curved leaves and viruses, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as tomato production hazards

Inactive Publication Date: 2013-03-06
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to climate warming and changes in the agricultural planting structure, Bemisia tabaci, the carrier of tomato yellow leaf curl virus, has broken out in northern China in recent years, making tomato yellow leaf curl virus unprecedented outbreaks in various parts of my country in recent years. Rapid spread and spread northwards, causing serious damage to tomato production

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  • Method for inoculating tomatoes with tomato yellow leaf curl viruses
  • Method for inoculating tomatoes with tomato yellow leaf curl viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, Optimization of Bemisia tabaci transmission time (seedling age)

[0035] The tomato variety Cyclamen 6, which is sensitive to tomato yellow leaf curl virus, was used as the test variety to determine the best time for the transmission of the virus by Bemisia tabaci. The specific experimental methods are as follows:

[0036] Tomato seeds are sown in flower pots with a diameter of 25 cm. When the first true leaf of the tomato grows, they are transplanted into plastic cups with a diameter of about 10 cm, and placed in an insect-proof net room for cultivation to avoid being infected by Bemisia tabaci or The virus infection was used for the Bemisia tabaci virus transmission experiment when it grew to 2, 3, and 4 true leaves, respectively.

[0037] Transplant the tomato seedlings into a plastic cup with a diameter of about 10 cm, slow down the seedlings for about 5 days, and when they grow to 2, 3, and 4 true leaves, select 20 to 30 plants with good growth condition...

Embodiment 2

[0040] Embodiment 2, the optimization of the population density of whitefly bemisia tabaci

[0041] The tomato variety Cyclamen 6, which is sensitive to tomato yellow leaf curl virus, was used as a test variety to determine the optimal population density of Bemisia tabaci. The specific experimental methods are as follows:

[0042] 1. Artificial inoculation of poisonous Bemisia tabaci on tomato seedlings

[0043]Tomato seeds are sown in flower pots with a diameter of 25 cm. When the first true leaf of the tomato emerges, they are divided according to the variety and transplanted into plastic cups with a diameter of about 10 cm. They are placed in an insect-proof net room for cultivation to avoid Infected by Bemisia tabaci or virus, when it grows to 4 true leaves, it is used for the experiment of Bemisia tabaci virus transmission.

[0044] Transplant the tomato seedlings into a plastic cup with a diameter of about 10 cm, slow down the seedlings for about 5 days, and when they g...

Embodiment 3

[0048] Example 3: To inoculate tomato with tomato yellow leaf curl virus and identify the resistance of tomato to tomato yellow leaf curl virus

[0049] 1. Basic Materials and Methods

[0050] 1.1 Tomato varieties and their seedling cultivation

[0051] As shown in Table 1, there are 13 tomato varieties including Cyclamen 6 and Jinpeng 1, and the tomato varieties are from the Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences;

[0052] Tomato seeds are sown in flower pots with a diameter of 25 cm. When the first true leaf of the tomato emerges, they are divided according to the variety and transplanted into plastic cups with a diameter of about 10 cm. They are placed in an insect-proof net room for cultivation to avoid Infected by Bemisia tabaci or virus, when it grows to 4 true leaves, it is used for the experiment of Bemisia tabaci virus transmission.

[0053] 1.2 Bemisia tabaci transmission (artificial inoculation of poisonous bemisia tabaci...

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Abstract

The invention discloses a method for inoculating tomatoes with tomato yellow leaf curl viruses. The method for inoculating the tomatoes with the tomato yellow leaf curl viruses provided by the invention comprises the steps that four-true-leaf tomato plants to be identified are put into 40-mesh net bags; one tomato plant to be identified is put into one net bag; Q-biotype Bemisia tabacis with the tomato yellow leaf curl viruses are put into the net bags; and six Q-biotype Bemisia tabacis with the tomato yellow leaf curl viruses are put into each net bag for virus inoculation and transmission, so that the inoculation of the tomato yellow leaf curl viruses is completed. With the adoption of the method for inoculating the tomatoes with the tomato yellow leaf curl viruses, the four-true-leaf tomato plants are inoculated with the tomato yellow leaf curl viruses respectively according to the density of six Q-biotype Bemisia tabacis per plant; the plants sensitive to the tomato yellow leaf curl viruses are in morbidity after one week since the inoculation; and after two weeks, the morbidity reaches 100%.

Description

technical field [0001] The invention relates to a method for inoculating tomatoes with tomato yellow leaf curl virus. Background technique [0002] Tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV), belongs to Geminiviridae (Geminiviridae) bean golden mosaic virus (Begomovirus) virus, because this genus virus can only be infected by whitefly (Bemisia tabaci) under natural conditions. tabaci) spread in a persistent manner, also known as whitefly-borne geminivirus. Due to climate warming and changes in the agricultural planting structure, Bemisia tabaci, the carrier of tomato yellow leaf curl virus, has broken out in northern China in recent years, making tomato yellow leaf curl virus unprecedented outbreaks in various parts of my country in recent years. Rapid spread and spread to the north, causing serious harm to tomato production. Therefore, the prevention and treatment of tomato yellow leaf curl virus disease is urgent. The application of disease-resi...

Claims

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Application Information

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IPC IPC(8): A01G7/06
Inventor 罗晨田兆丰刘伟成张帆卢向阳吴慧玲刘德文
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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