Centrifugal micro-fluidic device and method for immunoassay
A microfluidic device and immunoassay technology, which is applied in nanotechnology for sensing, measuring devices, biological testing, etc., can solve the problems of complex experimental steps and long test time, and achieve improved accuracy, sensitivity, and applicability Effect
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[0061] According to an exemplary embodiment, the first reaction chamber 300 of the microfluidic device may contain a single label conjugate comprising one labeling substance to detect one analyte; however, the first reaction chamber 300 may also contain different labeling substances. Several labeled conjugates for the simultaneous detection of multiple analytes. For example, for the simultaneous detection of four different types of analytes, four types of label conjugates containing four different lanthanide(III) chelates (e.g., Eu, Sm, Dy, and Tb) as separate labeling species substances may be contained together in the first reaction chamber 300.
[0062] An advantage of the configuration described above is that a single microfluidic structure 1000 can be used to detect multiple analytes. Simultaneous detection of multiple analytes is also possible by using only one microfluidic device with several microfluidic structures built into it. This configuration is superior to con...
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