Serum micro ribonucleic acid (miRNA) biomarker of bladder cancer and detection method of expression quantity thereof

A technology of biomarker and bladder cancer, applied in the fields of biotechnology and oncology, can solve the problems of serum research and development of patients without bladder cancer, and achieve the effect of great theoretical significance

Active Publication Date: 2013-02-13
NANJING MEDICAL UNIV
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  • Serum micro ribonucleic acid (miRNA) biomarker of bladder cancer and detection method of expression quantity thereof
  • Serum micro ribonucleic acid (miRNA) biomarker of bladder cancer and detection method of expression quantity thereof
  • Serum micro ribonucleic acid (miRNA) biomarker of bladder cancer and detection method of expression quantity thereof

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[0020] The present invention will be described in detail below in conjunction with the examples. This example is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and processes are provided, but the scope of the present invention is not limited to the following examples. The conditions and methods not indicated in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer.

[0021] 1. Sample collection and RNA extraction

[0022] Fifty-six new cases of bladder cancer and 60 age- and sex-matched controls were collected. The cases are mainly collected by the hospital and have been pathologically diagnosed. Controls were from healthy non-tumor patients. All subjects signed informed consent and provided 200uL serum.

[0023] 10 pairs of age-sex-matched cases and controls were randomly selected, and the total RNA in serum was extracted with QIANGEN kit to m...

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Abstract

The invention relates to a serum micro ribonucleic acid (miRNA) biomarker used for detection of bladder cancer, overcomes the defects in the prior art, provides a miRNA marker circulating in serum of a patient having the bladder cancer, establishes a blood circulation miRNA expression profile of the patient having the bladder cancer, discloses the value of serum miRNAs in diagnosis of the bladder cancer, and provides support for early detection and early treatment of the patient having the bladder cancer clinically. According to the serum miRNA biomarker of the bladder cancer and a detection method of expression quantity of the serum miRNA biomarker, for the first time, the serum miRNAs, especially miR-663b and miR-497 can be used as an important indicator of biological detection and plays roles in clinical early diagnosis of the bladder cancer, differential diagnosis and effective observation, a theoretical basis is provided for research on the serum miRNAs of urinary system tumor in the future, novel ideas are provided for the diagnosis of the bladder cancer at the molecular level, and the serum miRNA biomarker of the bladder cancer and the detection method of expression quantity of the serum miRNA biomarker have great theoretical significance and potential practical value.

Description

technical field [0001] The invention belongs to the fields of biotechnology and oncology, and in particular relates to a method for detecting serum miRNA biomarkers and a method for detecting expression levels of bladder cancer. Background technique [0002] Bladder cancer is an important public problem. In the world, the incidence of bladder cancer ranks fourth among malignant tumors, and it is one of the most common malignant tumors of the urinary system in my country. Clinically, cystoscopy and urine exfoliation cytology are still the irreplaceable gold standard for the diagnosis of bladder cancer, but cystoscopy will bring trauma and expensive detection costs to patients, while cytology has low sensitivity. Therefore, the research on molecular biology of bladder cancer has been paid more and more attention at home and abroad, hoping to reveal the occurrence, development, metastasis and prognosis of bladder cancer at the molecular level, so as to improve the diagnosis and...

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Application Information

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IPC IPC(8): C12N15/113C12Q1/68G01N21/64
Inventor 张正东王美林石丹妮储海燕马兰仝娜吴冬梅
Owner NANJING MEDICAL UNIV
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