Cell culture insert

Abstract

The invention relates to a cell culture insert, wherein two different types of cells can separately and independently grow into cell populations in the same container, and then the two cell populations grow to contact each other to form a cell junction area through the cell culture insert. The cell culture insert is a transparent container, and comprises a side wall (1) and a bottom wall (2), wherein the top end of the side wall (1) is distributed with a plurality of outwardly extending side wings (3), and the bottom wall (2) comprises a platform portion (4) positioned on the middle of the bottom wall (2), arc surfaces (5) positioned on both sides of the platform portion (4), and slope-like planes (6). Two types of cells are respectively inoculated onto both sides of the platform portion (4) by a researcher, a liquid level of a culture medium is lower than the platform portion (4), the two types of the cells can grow in an isolated manner, after the cells adhere to the wall to form cell populations, the culture medium is added to the liquid level of higher than the platform portion (4), the two types of the cells grow and extend toward the dome plane, when the two types of the cells contact each other, biological behavior conditions of the two types of the cells on the dome plane cell junction area can be observed through a microscope when the two types of the cells interact.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a culture container used for culturing cells in biological science research. In particular, two different cells can be isolated and cultured in the same container to make them adhere to and grow separately to form a cell group, and then make the two cell groups Continue to grow until they touch each other and form a cell culture pool with a clear cell junction area. Background technique [0002] At present, the bottoms of cell culture containers such as culture flasks, culture plates, culture tanks, and culture dishes used to culture cells in biological science research are all flat. When researchers inoculate a certain type of cell in the culture container, the inoculated cells It is injected into the culture container together with the liquid culture medium. The cells are suspended in the culture medium. Therefore, the cells inoculated into the culture container will be evenl...

AI Technical Summary

Problems solved by technology

However, when it is necessary to study the interaction between two different types of cells, if the two types of cells to be studied are directly inoculated into a common flat-bottomed cell culture vessel, the two types of cells will be mixed with each other and evenly distributed In the culture container, researchers can only obtain the situation when two different types of cells are mixed with each other and grow, but cannot obtain two kinds of cells that first grow independently into cell groups and then continue to grow until they contact each other and form a clear pattern. Conditions at the junctional zone of cell populations

That is to say, it is impossible to study the biological behavior of the cells at the junction of the two cell populations when they are in contact with each other using common flat-bottomed cell culture vessels, which limits cell biology research

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