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Method for building bamboo reed tissue culture system

A technique of tissue culture and system, applied in the field of plant tissue culture, can solve difficult problems such as genetic transformation, achieve the effects of strengthening the ability to adapt to the external environment, growing faster, and improving the survival rate of transplanting

Inactive Publication Date: 2014-04-02
HUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Miguel reported in 2012 the research on the tissue culture of Arundis, but it is difficult to use it for genetic transformation in terms of explant materials and propagation methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for establishing a reed bamboo tissue culture system, comprising the steps of,

[0028] (1) Sterilize the explants. The axillary buds of Phyllostachys rex are taken as explants. The height of the axillary buds is 4-6 mm. In the ultra-clean bench, put 75% alcohol into the container until the explants are completely submerged, discard the alcohol solution after 10 seconds, and immediately add 0.2% mercuric chloride solution to the explant container until all the explants are submerged, and place for 10 Minutes later, the explants were shaken and rinsed 5 times with sterile water, each time for one minute.

[0029] (2) Primary culture. Under aseptic conditions, 2-3 mm of the tissue with the top growth point was stripped and inserted into the culture medium to induce adventitious buds. The medium is: add 30 g / L sucrose, 8 g / L agar to MS, then add 5 mg / L 6-BA and 0.5 mg / L IBA, and finally adjust the pH to 5.8; the culture temperature is: 25 ± 1 ℃, The light inte...

Embodiment 2

[0034] A method for establishing a reed bamboo tissue culture system, comprising the steps of,

[0035] (1) Sterilize the explants. The axillary buds of Phyllostachys rex are taken as explants. The height of the axillary buds is 4-6 mm. In the ultra-clean bench, put 75% alcohol into the container until the explants are completely submerged, discard the alcohol solution after 10 seconds, and immediately add 0.2% mercuric chloride solution to the explant container until all the explants are submerged, and place it for 10 seconds. Minutes later, the explants were shaken and rinsed 5 times with sterile water, each time for one minute.

[0036](2) Primary culture. Under aseptic conditions, 2-3 mm of the tissue with the top growth point was stripped and inserted into the culture medium to induce adventitious buds. The medium is: add 30 g / L sucrose, 8 g / L agar to MS, then add 5 mg / L 6-BA and 0.5 mg / L IBA, and finally adjust the pH to 5.8; the culture temperature is: 25 ± 1 ℃, The...

Embodiment 3

[0041] A method for establishing a reed bamboo tissue culture system, comprising the steps of,

[0042] (1) Sterilize the explants. The axillary buds of Phyllostachys rex are taken as explants. The height of the axillary buds is 4-6 mm. In the ultra-clean bench, put 75% alcohol into the container until the explants are completely submerged, discard the alcohol solution after 10 seconds, and immediately add 0.2% mercuric chloride solution to the explant container until all the explants are submerged, and place it for 10 seconds. Minutes later, the explants were shaken and rinsed 5 times with sterile water, each time for one minute.

[0043] (2) Primary culture. Under aseptic conditions, 2-3 mm of the tissue with the top growth point was stripped and inserted into the culture medium to induce adventitious buds. The medium is: add 30 g / L sucrose, 8 g / L agar to MS, then add 5 mg / L 6-BA and 0.5 mg / L IBA, and finally adjust the pH to 5.8; the culture temperature is: 25 ± 1 ℃, Th...

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PUM

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Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a method for building a bamboo reed tissue culture system. The method comprises the steps of (1) explant disinfection, wherein axillary buds of bamboo reeds are fully soaked in 75% of ethanol for 10 seconds and then moved into 0.2% of mercury bichloride solution for 10 minutes; (2) primary culture, wherein a culture medium is formed by adding cane sugar 30g / L and agar 8g / L in MS, then adding 6-BA 5mg / L and IBA 0.5mg / L and finally adjusting potential of hydrogen (pH) to 5.8; (3) secondary culture, wherein a culture medium and culture conditions of the secondary culture are the same as the culture medium and culture conditions for the primary culture; (4) strong bud and root culture, wherein the buds are cultured in the secondary culture medium for one month to finish strong bud culture and then planted in a rooting culture medium, and the rooting culture medium is formed by adding IBA 0.5 mg / L into the MS; and (5) domestication and transplantation, wherein the domestication is performed by using a floating bed domestication system for one week and then transplantation is performed. By means of the method, the efficient breeding of the bamboo reeds can be achieved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a reed bamboo tissue culture system. Background technique [0002] Plant tissue culture is a technique established on the basis of the theory of cell totipotency. In recent years, as a basic experimental technique and basic research method, plant tissue culture has shown great application potential and has been widely used in various fields such as botany, genetics, and breeding. my country's current rapid economic development is facing relatively large challenges in terms of energy and pollution. Arundis is considered an herbal energy plant that also has pollution remediation properties. Arundis to Cu 2+ , pb 2+ 、Cd 2+ , Zn 2+ 、Ni 2+ , Hg 2+ 、Cr 6+ Heavy metals such as cadmium and mercury have a certain tolerance and are used to remediate wetlands polluted by heavy metals such as cadmium and mercury. The research shows...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 杨志红周莉凡韩志萍
Owner HUZHOU TEACHERS COLLEGE
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