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Accurate correction method of fluorescence quenching rate in inner filtering efficiency process of fluorescence quenching system

A technology of fluorescence quenching and correction method, which is applied in the field of fluorescence spectrum analysis, and can solve problems such as restricting the application of fluorescence quenching method, falsely high fluorescence quenching rate, and inability to accurately correct fluorescence quenching rate data.

Inactive Publication Date: 2013-02-06
HEFEI UNIV OF TECH
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Problems solved by technology

[0005] The influence formed by the absorption distribution and the influence of the fluorescence inner filter effect are accompanied and appear at the same time, which jointly cause the artifact of the falsely high fluorescence quenching rate
At present, the existing correction technology only corrects the fluorescence inner filter effect separately, but ignores the influence of the absorption distribution that occurs at the same time, so it is fundamentally impossible to accurately correct the obtained fluorescence quenching rate data and obtain a true The quenching rate restricts the application of fluorescence quenching method

Method used

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  • Accurate correction method of fluorescence quenching rate in inner filtering efficiency process of fluorescence quenching system
  • Accurate correction method of fluorescence quenching rate in inner filtering efficiency process of fluorescence quenching system
  • Accurate correction method of fluorescence quenching rate in inner filtering efficiency process of fluorescence quenching system

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Embodiment

[0046] Example: Pentathiophene (5T) and fullerene (C 70 ) for the precise correction of the fluorescence quenching spectrum

[0047] Absorption and fluorescence spectra of 5T and C 70 There is a large degree of overlap between the absorption spectra of the two, and the mixture does not form a ground state complex. After being excited, part of the photoinduced electrons at 5T will go to the C 70 transfer, resulting in fluorescence quenching. The solvent adopts o-dichlorobenzene, which is transparent and non-absorbing in the range of ultraviolet / visible light.

[0048] UV-2550 ultraviolet-visible light absorption spectrometer of Shimadzu Corporation was used for the measurement of absorption spectrum; F-4500 fluorescence spectrometer of Hitachi Corporation was used for the detection of fluorescence spectrum, equipped with a constant temperature water bath; the experiment was completed at 25°C.

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Abstract

The invention discloses an accurate correction method of a fluorescence quenching rate in an inner filtering efficiency process of a fluorescence quenching system, and the method is characterized by comprising the following steps of: firstly, correcting influences of competitive absorption on a light spectrum; then, correcting the influences of absorption on the light spectrum; and correcting the influences of absorption distribution on the light spectrum. The method disclosed by the invention can be used for carrying out combined correction on the influences caused by a fluorescence inner filtering efficiency and absorption distribution, so as to ensure that the light spectrum of the quenching system is accurately corrected; and the fluorescence quenching rate in the quenching process is really reflected.

Description

technical field [0001] The invention relates to fluorescence spectrum analysis, in particular to a spectrum analysis method in fluorescence quenching test under the condition of fluorescence inner filter effect. Background technique [0002] Fluorescence analysis is a method for substance identification and content determination based on the fluorescence spectrum of the substance (including parameters such as intensity and shape), among which the fluorescence quenching method can be used to analyze the content of some substances whose autofluorescence is difficult to detect but has quenching characteristics , can also be used to analyze the energy transfer or electron transfer between two substances (that is, between the fluorescent substance and the quencher) during the fluorescence quenching process. [0003] For the fluorescence quenching system, when there is a serious overlap between the absorption spectrum and the corresponding fluorescence spectrum of the fluorescent ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 陈向东吴本科高峰袁自钧程萍王飞杨继平
Owner HEFEI UNIV OF TECH
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