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Collagen scaffold material for cornea

A collagen and composite scaffold technology, applied in the field of medical biomaterials, can solve the problems of low success rate of surgery and difficult source of corneal donors

Inactive Publication Date: 2013-02-06
TIANJIN SAIRUI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional corneal transplantation has two major disadvantages. One is the difficulty in obtaining corneal donors, and the other is the low success rate of the operation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Take 1.0 g of commercially available chitosan hydrochloride, slowly pour it into 100 ml of collagen solution with a concentration of 5.0%, and pour it while stirring. Wherein the collagen solution is a mixed collagen of type I collagen and type III collagen dissolved in 0.2mol / L hydrochloric acid. After stirring evenly, stir for 1 hour at an ambient temperature of 23°C. Adjust the pH value to 7 with 1.0mol / L sodium hydroxide solution. The obtained composite material is laid and cross-linked to form a film. The mass ratio of polysaccharide to collagen is 1:5;

Embodiment 2

[0012] Take 1.3 g of commercially available sodium alginate, slowly pour it into 100 ml of collagen solution with a concentration of 13.0%, and pour it while stirring. Wherein the collagen solution is a mixed collagen of type I collagen and type II collagen dissolved in 3.0 mol / L hydrochloric acid. After stirring evenly, stir for 7 hours at an ambient temperature of 23°C. Adjust the pH value to 6 with 5.0mol / L sodium hydroxide solution. The obtained composite material is laid and cross-linked to form a film. The mass ratio of polysaccharide to collagen is 1:10.

Embodiment 3

[0014] Take 1.5 g of commercially available carboxylated chitosan, slowly pour it into 100 ml of collagen solution with a concentration of 1.0%, and pour it while stirring. Wherein the collagen solution is a mixed collagen of type I collagen and type III collagen dissolved in 0.2mol / L hydrochloric acid. Stir evenly and stir for 12 hours at an ambient temperature of 38°C. Adjust the pH value to 7 with 1.0mol / L sodium hydroxide solution. The obtained composite material is laid and cross-linked to form a film. The mass ratio of polysaccharide to collagen is 3:2.

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Abstract

The invention discloses a scaffold material for clinical cornea reconstruction and a preparation method of the material, belongs to the field of medical biomaterials, and mainly aims at solving the present problems that the clinical supply of cornea donors is difficult and the success rate of operations is low. The cornea is reconstructed by providing a keratocyte growth matrix for a cornea receptor. The material prepared by the method is prepared by adjusting pH values of polyose and a collagen solution to be within a certain range at a certain environmental temperature and then conducting crosslinking membrane forming. The material is stable, uniform, transparent, water-proof and pressure-resistant, and is good in biocompatibility. The cornea reconstruction of the corneal transplantation receptor is facilitated.

Description

technical field [0001] The invention relates to a composite material of polysaccharide and collagen and a preparation method thereof. This material has a certain degree of light transmission, and its appearance is film-like. Corneal cell culture scaffolds used in ophthalmology prior to corneal transplantation. It belongs to the field of medical biomaterials. Background technique [0002] At present, the blindness of more than 10 million people in the world is caused by corneal disease, which is the second leading cause of blindness, second only to cataract. 80% of them can be relieved of blindness through corneal transplantation. However, traditional corneal transplantation has two major disadvantages. One is the difficulty in obtaining corneal donors, and the other is the low success rate of the operation. [0003] In view of this, it is particularly important to develop a scaffold material for the survival and reproduction of patient-specific corneal cells before kerat...

Claims

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Application Information

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IPC IPC(8): A61L27/26
Inventor 张春红王宗龙万力
Owner TIANJIN SAIRUI BIOLOGICAL TECH
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