Method for creating high-frequency regeneration system of zoysia japonica

Abstract

The invention discloses and provides a method for creating a high-frequency regeneration system of zoysia japonica. The method comprises the followings steps that a single zoysia japonica seed is sequentially subjected to induction culture, first generation subculture, second generation subculture and third generation subculture to obtain the high-frequency regeneration system of the zoysia japonica. The experiments prove that the invention provides the method for creating a high-frequency regeneration callus system or a regeneration plant and a special culture medium. According to the invention, Japanese zoysia japonica varieties are screened, a single seed is taken as an explant, different callus systems are respectively induced, then the callus system with high differentiative capacity is screened after proportioning combination of the culture medium and desiccation subcultures for multiple times, and a large quantity of high-frequency regeneration calluses propagated in each system are homogeneous. Finally, the method for creating the high-frequency regeneration system of zoysia japonica is built to provide a practical method to improve the genetic transformation efficiency and the tissue culture regeneration rate of zoysia japonica.

Description

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AI Technical Summary

Benefits of technology

This patented process allows researchers to study how plants develop new properties by selecting specific types from their seed sources. By combining these materials together they can produce more desirable results than just mixing them separately. They also discovered that certain parts (called zygosity factors), called regulators, control this developmental pathway differently depending upon what type of material was chosen. These findings help scientists better identify ways to improve crop production through breeding techniques such as crossing pollenizers between two species's reproductive organs.

Problems solved by technology

This patented technical solution describes how zebytes can be grown into rootlets without being hardened during their growth process due to lack of nutritional sources needed for roots. Existing techniques involve growing seedlings directly onto slabs containing cultured cells called zygotes, resulting in difficulties in acquiring parentage. Additionally, current regulatory mechanisms limit catheterisirms usage and require expensive equipment. There exists a challenge associated with finding effective ways to promote vegetative propagations while maintaining competence over generational change.

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