Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Minicircle gene vector and its preparation method and use

A gene carrier and micro-circle technology, which can be used in gene therapy, the introduction of foreign genetic material using vectors, and pharmaceutical formulations, etc., can solve the problem of low target cell efficiency.

Active Publication Date: 2013-01-23
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, MC also has the problem of low delivery efficiency to target cells in vivo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Minicircle gene vector and its preparation method and use
  • Minicircle gene vector and its preparation method and use
  • Minicircle gene vector and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] The microcircle gene carrier and its preparation method and application will be further explained below in conjunction with the accompanying drawings and examples.

[0048] A microcircle gene carrier is provided, and after the microcircle gene carrier is sent into target cells infected with viruses, viral gene therapy vectors can be produced. Target cells include hepatocytes in mammals, nonparenchymal hepatocytes such as liver endothelial cells, Kuffer cells or cells of other organs.

[0049] The produced viral gene therapy vectors can infect new target cells like wild viruses, produce new infectious recombinant viruses, and can express therapeutic transgene products themselves, so as to play a long-term and effective role and become a safe , Efficient, convenient and economical gene therapy technology.

[0050] This minicircle gene vector does not contain the backbone DNA components in standard plasmids that can turn off transgene expression, including: promoters, com...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a minicircle gene vector and its preparation method and use. The minicircle gene vector does not contain a backbone DNA ingredient which can close transgene expression and is in a standard plasmid. The minicircle gene vector comprises a promoter, an ingredient for gene expression and virus replication in a viral genome, and a transgene or a transgene expression box for expression of a treatment transgenosis product. The ingredient for gene expression and virus replication in a viral genome is cloned into a vector so that the combined chimeric minicircle gene vector is obtained. After being fed into a target cell infected by viruses, a small amount of the minicircle gene vectors can produce recombinant viruses having treatment and infection effects. Through infecting a new target cell, the recombinant viruses produce new recombinant viruses having infection effects, and finally, the recombinant viruses diffuse to all target cells. Compared with the traditional gene vectors, the minicircle gene vector can be efficiently delivered into a target cell and has strong and lasting treatment effects. The invention also provides a preparation method and a use of the minicircle gene vector.

Description

【Technical field】 [0001] The invention relates to the field of gene therapy, in particular to a microcircle gene carrier and its preparation method and application. 【Background technique】 [0002] Gene therapy is the most ideal way to treat many hereditary and acquired diseases, and there have been many successful reports in preclinical experiments. However, clinically successful cases are rarely reported. The core of gene therapy is to deliver therapeutic vectors into target cells, but there are still several technical problems to be solved in terms of vector construction and delivery. Gene therapy vectors can be divided into two categories, namely viral vectors and non-viral vectors. Viral vectors have a high efficiency of infecting cells, but due to their immunogenicity and insertional mutations, they are prone to cause severe reactions, including carcinogenicity and fatality, so they are difficult to be accepted clinically; in addition, high manufacturing costs are als...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/66A61K48/00A61P31/20A61P1/16
Inventor 陈志英何成宜
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com