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Alpha fetoprotein heteroplasmon isolation kit for liver cancer diagnosis, composition reagents of kit and application

A technology of alpha-fetoprotein and kit, applied in the direction of analysis materials, biological testing, material inspection products, etc., can solve the problems affecting the capture efficiency and sensitivity, and achieve the effect of improving specific and efficient separation and purification effects

Active Publication Date: 2014-11-05
同昕生物技术(北京)有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the uniformity of the magnetic particles themselves, surface hydrophobicity, tension, and surface coating proteins, aggregation and sedimentation will occur in the actual application process, which will affect the capture efficiency and sensitivity of lectin-coated magnetic beads to AFP-L3 , to be improved

Method used

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  • Alpha fetoprotein heteroplasmon isolation kit for liver cancer diagnosis, composition reagents of kit and application
  • Alpha fetoprotein heteroplasmon isolation kit for liver cancer diagnosis, composition reagents of kit and application
  • Alpha fetoprotein heteroplasmon isolation kit for liver cancer diagnosis, composition reagents of kit and application

Examples

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Embodiment Construction

[0049] The following examples serve to further illustrate the present invention in detail.

[0050] Reagents used:

[0051] Active magnetic beads (MB): Invitrogen, Dynal-Myone

[0052] 25mM MES: 2-(N-morpholino)ethanesulfonic acid (sigma, M3671) buffer solution, weigh 4.88g MES in 900mL ultrapure water, adjust the pH to 5.0 after complete dissolution, and dilute to 1L.

[0053] EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide) solution: sigma, 39391

[0054] Eluent: 10M fucose eluent (Sigma, F2252)

[0055] Lentil agglutinin LCA: 1 mg / ml, dissolved in 25 mM MES buffer, pH 5.0 (Sigma-L9627);

[0056] Reaction solution: 10mM pH7.4 Tris-HCl buffer containing 0.05% (w / v) Tween-20

[0057] pH7.510mM PBS: Weigh 0.27g potassium dihydrogen phosphate (KH2PO4) (Sinopharm, 10017608), 1.42g disodium hydrogen phosphate (Na2HPO4) (Sinopharm, 100203008), 8g sodium chloride (NaCl) (Sinopharm, 10019308), Dissolve 0.2g of potassium chloride (KCl) (Sinopharm, 10016308) in 900mL of ultrapu...

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Abstract

The invention relates to an alpha fetoprotein heteroplasmon isolation kit for liver cancer diagnosis, composition reagents of the kit and the application and relates to the field of medical reagents. In a preferable implementation mode, uniform and stable protection liquid is successfully obtained, and stable, high-binding-force and specific lectin coated magnetic beads, uniform and stable lectin-magnetic bead solution and optimal reaction solution and eluent are obtained by optimizing the kit.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and specifically relates to an alpha-fetoprotein heterogeneous separation kit for liver cancer diagnosis based on magnetic particle separation technology, as well as its component reagents and application. Background technique [0002] It is generally believed that alpha-fetoprotein (AFP) is a relatively specific tumor marker for HCC (hepatocellular carcinoma, hepatocellular carcinoma), and sustained elevation of AFP is a risk factor for HCC. At present, some European and American scholars believe that the sensitivity and specificity of AFP are not high. The 2010 edition of the American Society for the Study of Liver Diseases (AASLD) guidelines no longer use AFP as a screening indicator. However, most HCCs in my country are related to HBV (hepatitis B virus) infection. Different from the pathogenic factors of HCC in western countries (mostly HCV, alcohol and metabolic factors), combined ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/531G01N33/68
Inventor 李全焦守恕肖智
Owner 同昕生物技术(北京)有限公司
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