Primer system for PCR (polymerase chain reaction) identification for deer, pig, cow, sheep, horse, donkey, rabbit and chicken

Abstract

The invention discloses a primer system for PCR (polymerase chain reaction) identification for a deer, a pig, a cow, a sheep, a horse, a donkey, a rabbit and a chicken, and can detect eight animal species in one time so as to achieve a purpose that common animal species can be almost covered. The primer of the primer system only carries out specific amplification on a respective species target segment without reacting with other species, and therefore the primer can be suitable for multiple PCR reaction characterized in that at least two primer pairs are induced in one-time PCR reaction so as to effectively shorten experiment time. In addition, when the PCR is carried out, an optimized specific PCR reaction system and reaction program can be used, a detection flow is simplified due to the adoption of a uniform PCR detection method, a quick and efficient identification mode with the high specificity is established so as to effectively identify whether the deer blood product is true and false and identify the adulteration mode, and a modern molecular biology detection means is provided for deer blood quality control. In addition, the primer system can be cooperated with relevant reagent to be prepared into a kit, thereby being convenient to use. Meanwhile, possibility is provided for the industrial production and application, and the primer system has an excellent application prospect.

Description

technical field [0001] The present invention relates to the field of molecular biology, in particular to a primer system for PCR identification of deer, pig, cow, sheep, horse, donkey, rabbit and chicken and a PCR identification method, which is applicable to the rapid detection and identification of the above eight kinds of animal blood products, It is especially suitable for rapid anti-counterfeiting identification of deer blood products. Background technique [0002] Deer blood is the blood of deer family animals. According to the "Compendium of Materia Medica": "Deer blood can nourish deficiency and damage, benefit essence and blood, relieve acne poison and drug poison." As a traditional precious Chinese medicinal material, deer blood is irreplaceable. Its medicinal value has been widely recognized and developed in depth. The product line has been continuously enriched, including deer blood wine, fresh deer blood, deer blood crystal, etc. The market value generated and ...

AI Technical Summary

Problems solved by technology

However, the above scheme does not design primers for deer, so although it can be determined whether the sample contains these four types of substances, it cannot be determined whether it is a deer product

[0005] From the above representative research results, it can be seen that these tests have the following shortcomings: (1) The detection range is narrow, and only a small number of species can be detected at a time, basically no more than four

This is because in the same total DNA template system, the greater the number of primer pairs, the more serious the interference between the primer pairs and the total DNA template, and the less guaranteed the specificity of the PCR reaction; Rapid and highly specific primer systems and detection methods for common species, which can also cause missed detections

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