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Longshan lily high-efficient detoxification process

A high-efficiency technology for Longshan lily, which is applied in the field of high-efficiency detoxification technology for Longshan lily, can solve the problems of reducing the survival rate of plants

Active Publication Date: 2014-01-08
湘西龙山农业科技园区管理委员会
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Problems solved by technology

However, if the temperature is too high, the survival rate of the plant will be reduced.

Method used

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Embodiment

[0031] A kind of Lily Longshan efficient detoxification process steps are as follows:

[0032] 1) Pretreatment: Rinse the collected Longshan lily bulbils to remove impurities such as sediment, and then sterilize after heat treatment at a constant temperature of 35°C.

[0033] 2), the first detoxification: take out the bulbils after constant temperature heat treatment, wash the impurities on the surface, peel off 0.4-0.5mm shoot tips without leaf primordium or with 1 leaf primordium, and inoculate them in the starting medium. When the induced bulblets reach a certain base number, proceed to the next step.

[0034] 3), the second detoxification: first place the culture medium with the small bulbs just transferred to culture at a constant temperature of 35°C to achieve the purpose of inactivating the virus; then use a dissecting needle to peel off 0.2-0.3mm in size without leaf primordia Inoculate in the starting medium; light culture temperature 25 ℃, light intensity 2500Lx, wh...

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Abstract

The invention discloses a Longshan lily high-efficient detoxification process, which is characterized in that the process comprises the following steps of: 1) pretreatment; 2) primary detoxification; 3) secondary detoxification; and 4) rooting culture. Through multi-generation detoxification combined with heat treatment, the invention ensures detoxification efficiency of 100%, and breaks through the problem of a low survival rate of tissue cultured seedlings in traditional detoxification process. An anti-oxidant AC is used for effective inhibition of the browning phenomenon for shoot tip culture. A certain amount of active carbon is added into an initial medium for effective adsorption of toxic substances such as phenols, aldehydes, and the like generated during the shoot tip breathing process, which reduces browning and improves the survival rate. 1 / 2 MS and high-concentration 6-BA are used in a pullulation induction medium, which can better promote the generation of bulblets and inhibit the growth of leaves; the concentration of active carbon is improved, which, on one hand, reduces browning and increases the survival rate, and on the other hand, forms dark environment to promote the generation of bulblets.

Description

Technical field: [0001] The invention relates to a high-efficiency detoxification process for Longshan lily, in particular to a new detoxification technology with extremely high detoxification efficiency for Longshan lily. Background technique: [0002] Longshan County in Western Hunan is known as the "Hometown of Lilies" in China, with a stable planting area of ​​more than 50,000 mu per year, accounting for 1 / 6 of the country's planting area. It is the county with the most lilies in the country. The production of lily is mainly based on asexual reproduction such as cutting propagation and ball propagation. For a long time, the seedlings of Longshan lily have basically been kept by farmers, and the variety has degraded seriously. In recent years, lily virus disease has been widespread in all lily producing areas in the county, and the infection rate has reached 100%. The yield and quality are declining year by year, which seriously restricts the development of lily industry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 颜芳燕周晓波向德高丁茁荑张治国吴艺飞白占兵
Owner 湘西龙山农业科技园区管理委员会
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