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Electrochemical biosensor for detecting oxalic acid concentration and its preparation and application

A biosensor and electrochemical technology, applied in the field of preparation of electrochemical biosensors, can solve problems such as electrochemical biosensors that are not prepared for oxalic acid detection, and achieve the effect of wide application prospects

Active Publication Date: 2015-09-09
上海碳源汇谷新材料科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

No electrochemical biosensors for oxalic acid detection have been prepared by taking advantage of the excellent electrochemical properties of graphene

Method used

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  • Electrochemical biosensor for detecting oxalic acid concentration and its preparation and application
  • Electrochemical biosensor for detecting oxalic acid concentration and its preparation and application
  • Electrochemical biosensor for detecting oxalic acid concentration and its preparation and application

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Embodiment 1

[0021] Partial reduction oxidation Graphene. Add 20 mg of ascorbic acid to 10 mL of 1 mg / mL graphene oxide aqueous solution, and place at room temperature for 2 h, 4 h, and 12 h, respectively. Then, it was filtered and washed through a microporous membrane, then dispersed in ultrapure water, and then dialyzed to obtain PCRGO with a concentration of 1 mg / mL. The samples obtained by reduction at different times were named CRGO-2, CRGO4, and CRGO-12, respectively.

[0022] Add 0.1mL, 1mg / mL CRGO-12 to 0.9ml 20mM sodium succinate buffer solution (pH3.5) containing 0.1-0.5mg oxalate oxidase and mix, and rotate and mix at 4°C for 2h. The mixture was centrifuged at 15000rpm for 10min, and thoroughly washed with buffer three times to remove free and weakly adsorbed OxOx molecules, and the precipitate was dispersed in 0.2ml of 20mM sodium succinate buffer solution (pH3.5) to form CRGO-12 A dispersion with a concentration of 0.5 mg / mL.

[0023] A glassy carbon electrode (3 mm in dia...

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Abstract

The invention relates to an electrochemical biosensor for detecting oxalic acid concentration, and preparation and application thereof. Oxalic acid oxidase is fixed on the surface of part of reduction-oxidation grapheme, a dispensing method is adopted to enable immobilized oxalic acid oxidase to be decorated on the surface of a glassy carbon electrode, and the electrochemical biosensor based on decoration of oxalic acid oxidase and part of reduction-oxidation grapheme is formed. The glassy carbon electrode serves as a working electrode, and a difference volt-ampere pulse method is adopted to detect the oxalic acid concentration. The biosensor has good electro-catalysis activity on oxalic acid. Along with increase of the immobilization amount, the electro-catalysis activity is reinforced. The detection range of the electrode is wide, and the electrode has low detection lower limit and responds rapidly.

Description

technical field [0001] The invention relates to the preparation of an electrochemical biosensor for detecting oxalic acid, in particular to an electrochemical biosensor based on partially reduced graphene oxide-immobilized oxalate oxidase, modified glassy carbon electrodes with the immobilized enzyme and its detection of oxalic acid . Background technique [0002] Oxalic acid is an organic substance that is produced in large quantities during cellular metabolism and is toxic to almost all organisms. Excessive accumulation of oxalic acid in the human body will lead to many diseases, such as hyperoxaluria, calcium oxalate stones, renal failure, cardiomyopathy and myocardial conductivity disorders. The determination of oxalic acid in urine and plasma is very important for the diagnosis and drug treatment of the first and second stages of hyperoxaluria. Analysis of oxalic acid in blood is very useful for early diagnosis of calcium oxalate stones. The diagnostic methods for ox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 郭守武张艳吴海霞杨海军
Owner 上海碳源汇谷新材料科技有限公司
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