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Two Strains of Motile Actinomycetes and Their Application in Anti-bacteria

A swimming actinomycetes and antibacterial technology, applied in the field of microorganisms, can solve the problems of low economic benefits in the pharmaceutical industry, and achieve the effect of small chemical synthesis

Active Publication Date: 2015-10-07
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because about 97% of the drugs produced by the pharmaceutical industry are imitated foreign varieties, although my country can be called a big country in pharmaceutical production, it is still a weak country in medical technology, which makes the overall economic benefits of the pharmaceutical industry low

Method used

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  • Two Strains of Motile Actinomycetes and Their Application in Anti-bacteria
  • Two Strains of Motile Actinomycetes and Their Application in Anti-bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of bacterial strain fermentation product

[0020] The fermentation product preparation process of bacterial strain is as follows: Actinoplanes sp.PDF-1 and PDF-2 pure culture are respectively inoculated to slant medium (yeast extract 0.4%, malt extract 1.0%, glucose 0.4%, agar 1.5%, pH7 .3) at 28°C for 10 days. Pick a 0.5 cm square piece of culture species from the slope and put it into 50ml fermentation medium (glucose 0.5%, malt extract 1%, cottonseed cake powder 1%, soluble starch 2%, yeast extract 0.5%, K 2 HPO 4 0.05%, (NH 4 ) 2 SO 4 0.5%, NaCl0.1%, CaCO 3 0.3%, pH7.2. ) in a 250ml Erlenmeyer flask at 28°C and 190 rpm on a rotary shaker for 4 days. Take 50ml of fermentation broth, extract it with 50ml of acetone, evaporate to dryness, and dissolve it with 5ml of DMSO.

Embodiment 2

[0021] Embodiment 2: verification of antibacterial activity

[0022] The agar dilution method was used to determine the antibacterial activity of the acetone crude extract from the fermentation broth. The tested strains included sensitive strains of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, sensitive strains of Staphylococcus epidermidis, and methicillin-resistant Staphylococcus aureus cocci, susceptible strains of Enterococcus faecalis, vancomycin-resistant E. Wait for 20 strains of bacteria.

[0023] 1. Prepare culture medium

[0024] The culture medium is Mueller-Hinton (MH) agar medium, which is prepared according to the product instructions, and has a pH of 7.2-7.4.

[0025] 2. Cultivation of tested strains

[0026] 20 tested strains were cultured overnight at 37°C.

[0027] 3. Preparation of drug-containing tablet

[0028] Add 1ml of fermented broth crude extract samples to 14ml of MH agar medium at a temperature of 45-50°C and mix well, th...

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Abstract

The invention provides two Actinoplanaceae strains and antibacterial application thereof. The Actinoplanaceae strains, Actinoplanes sp. PDF-1 and PDF-2, have preserving numbers of CGMCC No. 4692 and CGMCC No. 4693. The strains have effect in resisting bacteria, especially resisting drug-resistant bacteria. The strains provided by the invention are renewable microbe resources, can obtain products having activity in resisting drug-resistant bacteria, are suitable for large-scale industrial fermentation production, and have environmental pollution smaller than chemical synthesis.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to two strains of actinomycetes and their application in anti-bacteria. Background technique [0002] A recent survey showed that 65% of nosocomial bacterial infections in 47 American hospitals were caused by Gram-positive bacteria, the most important of which were Staphylococcus aureus and Enterococcus. With the widespread use of antibacterial drugs, the detection rate and drug resistance rate of Staphylococcus, especially methicillin-resistant Staphylococcus aureus (MRSA), are increasing year by year. Vancomycin, a glycopeptide antibiotic discovered in the 1950s, was used as the drug of choice for the treatment of multidrug-resistant Gram-positive bacterial infections due to its unique effects, and was once hailed as "human's response to intractable multidrug resistance." The last line of defense against Gram-positive infections." But this last line of defense is also in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P31/04C12R1/045A61K35/74
Inventor 余利岩张月琴董国霞李子强孙薇张玉琴李秋萍魏玉珍
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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