Method of extracting gutta-percha through enzymolysis
A technology of eucommia gum and enzymolysis is applied in the field of enzymatic hydrolysis and extraction of eucommia gum, which can solve the problems of long regeneration cycle of callus, low purity of gum silk, low extraction yield, etc., so as to improve solvent utilization efficiency and save process operation. time, the effect of improving filtration efficiency
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Embodiment 1
[0041] A preparation method of eucommia gum, which is made from natural eucommia ulmoides bark, eucommia leaves or eucommia samara skin, the molecular weight of eucommia ulmoides gum is 200,000-300,000, and the impurity content in eucommia gum is less than 0.5%. The specific preparation steps are as follows:
[0042] Step 1. Select Eucommia bark, wash it in clear water, and dry it so that the water content of Eucommia bark is less than 5%, and set aside;
[0043] Step 2: Pretreatment by enzymatic hydrolysis
[0044] A, prepare enzyme solution: take 0.27% cellulase, 0.01% pectinase and 0.02% protease to dissolve in water respectively by mass percentage, be mixed with the composite enzyme liquid containing cellulase, pectinase and protease;
[0045] B. Add the Eucommia bark cleaned in the above step 1 to the prepared compound enzyme solution containing cellulase, pectinase and protease at a material-to-liquid ratio of 1:1; stir at 50°C and 500r / min Under enzymatic hydrolysis fo...
Embodiment 2
[0051] A preparation method of eucommia gum, which is made from natural eucommia ulmoides bark, eucommia leaves or eucommia samara skin, the molecular weight of eucommia ulmoides gum is 200,000-300,000, and the impurity content in eucommia gum is less than 0.5%. The specific preparation steps are as follows:
[0052] Step 1. Select leaves of eucommia ulmoides, wash them in clear water, and dry them so that the water content in eucommia bark, eucommia leaves or eucommia seeds is less than 5%, and set aside;
[0053] Step 2: Pretreatment by enzymatic hydrolysis
[0054] A, prepare enzyme solution: take 0.27% cellulase, 0.01% pectinase and 0.02% protease to dissolve in water respectively by mass percentage, be mixed with the composite enzyme liquid containing cellulase, pectinase and protease;
[0055] B. Add the Eucommia ulmoides leaves cleaned in the above step 1 to the prepared compound enzyme liquid containing cellulase, pectinase and protease according to the ratio of materi...
Embodiment 3
[0061] A preparation method of eucommia gum, which is made from natural eucommia ulmoides bark, eucommia leaves or eucommia samara skin, the molecular weight of eucommia ulmoides gum is 200,000-300,000, and the impurity content in eucommia gum is less than 0.5%. The specific preparation steps are as follows:
[0062] Step 1, choose one of the eucommia samara skins, put into clear water, wash, and dry, so that the water content in the eucommia samara skins is less than 5%, and set aside;
[0063] Step 2: Pretreatment by enzymatic hydrolysis
[0064] A, prepare enzyme solution: take 0.27% cellulase, 0.01% pectinase and 0.02% protease to dissolve in water respectively by mass percentage, be mixed with the composite enzyme liquid containing cellulase, pectinase and protease;
[0065] B. Add the Eucommia ulmoides skin cleaned in the above step 1 into the prepared compound enzyme liquid containing cellulase, pectinase and protease at a material-to-liquid ratio of 1: 2; Enzymolysis ...
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