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Identification method of salt tolerance of barley

An identification method and a technology of salt tolerance, applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve difficult statistical analysis, differences between salt tolerance and adult plant salt tolerance, and large differences, etc. problems, to improve screening efficiency and reduce experimental errors

Inactive Publication Date: 2012-12-26
YANGZHOU UNIV
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Problems solved by technology

The former mainly determines the salt tolerance of barley through the growth of barley in natural saline soil. However, due to the uneven salt content in saline soil under natural conditions, the identification results between repetitions often have large differences, and it is difficult to carry out statistical analysis; the latter mainly has germination period. and salt tolerance identification at the seedling stage and artificially controlled saline soil pot identification, but there are certain differences between the germination and seedling salt tolerance and the adult plant salt tolerance, and the artificial control salt saline soil pot identification method is difficult to control the salinity. Consistent, and the test scale should not be too large, it is not suitable for large-scale germplasm resource screening and variety breeding

Method used

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  • Identification method of salt tolerance of barley
  • Identification method of salt tolerance of barley
  • Identification method of salt tolerance of barley

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Embodiment Construction

[0017] Salt tolerance identification using the doubled haploid population constructed from Japanese barley variety Nasonijiao and Jiangsu barley variety Taixing 9425:

[0018] 1) Use a 1.8×0.5×0.02-meter extruded board as the germination bed, drill holes with a hole spacing of 0.1 meter×0.1 meter, the hole diameter is 0.03m, and sew a 100-mesh nylon screen on the bottom of the board, and number them horizontally. Two replicates were set up for each control and treatment, and two parents were inserted every 50 families in each replicate; the variety CM72 was used as the salt-tolerant control, and the variety Gairdner was used as the salt-intolerant control;

[0019] 2) Divide the numbered extruded panels into two pools, and put two repetitions in each pool;

[0020] 3) Evenly pad 4 layers of absorbent paper in each hole of the extruded board, and sow 2 seeds in each hole;

[0021] 4) Fill each pool with tap water at a depth of 1-2cm. After the absorbent paper is fully absorbed...

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Abstract

The invention relates to identification, screening and variety breeding of crop genetic resources, and particularly relates to an identification method of salt tolerance of barley. The method comprises the steps of preparing a barley nutrient solution, preparing a barley germination substratum, germinating barley and identifying salt tolerance of barley. The method provided by the invention can ensure normal growth of barley during a growth period, avoid influence of environment difference for test results, reduce test errors and drastically improve screening efficiency of barley genetic resources.

Description

technical field [0001] The invention relates to identification, screening and variety selection of crop germplasm resources. Background technique [0002] Soil salinization is one of the most serious abiotic stresses in crop production. Currently, at least 20% of the cultivated land in the world is threatened by salinization to varying degrees. According to incomplete statistics, there are 3.8×10 8 hm Different degrees of salinized soil, China alone has salinized and secondary salinized land 4×10 7 hm or more, accounting for about 1 / 10 of the country's cultivated land, seriously affecting grain production and becoming the main factor restricting agricultural production. Screening and creating salt-tolerant germplasm and breeding salt-tolerant varieties to improve the adaptability of crops to saline environments is one of the important ways to reduce the harm of soil salinization and develop and utilize salinized land resources such as coastal tidal flats. Barley is one of...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/00A01G7/00
Inventor 吕超许如根周美学黄友圣
Owner YANGZHOU UNIV
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