Microfluidic chip for biological chemiluminescence detection and detection method thereof

Abstract

The invention discloses a microfluidic chip for biological chemiluminescence detection. The chip comprises an interface layer, a transparent layer, a channel layer, a reflecting layer and a fixed layer, which are sequentially arranged from top to bottom. The layers are connected by adhesives and fixed by fasteners; the interface layer and the transparent layer are respectively provided with a liquid inlet hole and a liquid outlet hole; the interface layer is also provided with a optical fiber interface; the channel layer is provided with a liquid inlet flow channel, a micro mixer, a detecting pool and a waste liquid buffer pool, which are successively communicate with each other; the optical fiber interface is arranged above the detecting pool; the bottom of the optical fiber interface is connected with a light penetration layer; the detecting pool is opened to the lower surface of the channel layer; the bottom of the detecting pool is connected with the reflecting layer; and the bottom of the waste liquid buffer pool is connected with the fixed layer. The invention also discloses a detection method of the microfluidic chip. The microfluidic chip improves reaction luminescence intensity of the detection solution and optical detection efficiency, and has advantages of simple preparation method, convenient operation, fast detection, high sensitivity, and accurate detection result.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a microfluidic chip for biochemiluminescence detection and a detection method thereof. Background technique [0002] At present, in the research of chemistry and life sciences, fluorescence and chemiluminescence are one of the most widely used high-sensitivity quantitative detection methods. Due to the high photon energy absorption efficiency of the fluorophore, the excited fluorophore in the molecule can release a large amount of light energy and generate a high-intensity light signal. Therefore, the pretreatment of the sample is simple and the detection is convenient. However, since the excited sample itself contains complex background fluorescent groups, non-specific excitation fluorescence will be generated, and the complex spectrum of the excitation light source will interfere with the detection of fluorescence light detectors. Therefore, various filter devices are requi...

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Problems solved by technology

[0004] At present, the microfluidic chips related to luminescence detection reported in the literature have the following disadvantages: 1. Rely on the microscope platform or the complex optical path composed of lenses and filters: such as the microfluidic chip disclosed in the Chinese patent application number 200910114403.8 The method for measuring substances in a single human red blood cell by chemiluminescence and the biochip fluorescence microspectral detection device and production method disclosed in the Chinese patent application number 201110153526.X; The efficiency is low, and the maximum luminous intensity cannot be achieved: such as the microfluidic chip for capillary electrophoresis separation and chemiluminescence detection disclosed in the Chinese patent application number 200910154432.7; 3. The luminescent area is completely transparent, and the scattered light in different directions is not fully utilized. Low light detection efficiency

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