Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sample diluent of oral mucosal transudate for detecting HIV antibody by colloidal gold method, and preparation method thereof

A technology for oral mucosa and exudate, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as not seen, and achieve the effect of reducing the reaction time

Inactive Publication Date: 2012-11-07
CHENGDU XIEHE BIOLOGICAL TECH
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method of using oral mucosal exudate as a sample to detect AIDS antibodies is still in its infancy, and there has been no report on a sample diluent specially used to detect HIV antibodies in oral mucosal exudate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Oral mucosal exudate colloidal gold method for detecting HIV antibody sample diluent, including the following components:

[0038] Raw material name Standard concentration ( / L)

[0039] Disodium hydrogen phosphate 17.3g

[0040] Sodium dihydrogen phosphate 2.9g

[0041] Sodium chloride 8.3g

[0042] 10% Tween 80 10.5ml

[0043] Bovine Serum 142.5ml

[0044] Avidin 28.5mg

[0045] Biotin 19ml

[0046] EDTA-2Na 21g

[0047] NaN3 2.9ml

[0048] The balance is purified water

[0049] The preparation method of the sample diluent for detecting HIV antibody by the colloidal gold method of the oral mucosa exudate comprises the following steps:

[0050] A Measure purified water in a stirrer beaker, and start stirring, wherein, the volume of purified water measured accounts for 0.6 times of the total volume of the diluent fixed solution;

[0051] B Measure disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, and 10% soil temperature 80 respectively...

Embodiment 2

[0064] Oral mucosal exudate colloidal gold method for detecting HIV antibody sample diluent, including the following components:

[0065]Raw material name Standard concentration ( / L)

[0066] Disodium hydrogen phosphate 19.2g

[0067] Sodium dihydrogen phosphate 2.6g

[0068] Sodium chloride 9.2g

[0069] 10% Tween 80 9.5ml

[0070] Chicken Serum 157.5ml

[0071] Avidin 31.5mg

[0072] Biotin 21ml

[0073] EDTA-2Na 19g

[0074] NaN3 3.2ml

[0075] The balance is purified water

[0076] The preparation method of the sample diluent for detecting HIV antibody by the colloidal gold method of the oral mucosa exudate comprises the following steps:

[0077] A Measure purified water in a stirrer beaker, and start stirring, wherein, the volume of purified water measured accounts for 0.6 times of the total volume of the diluent fixed solution;

[0078] B Measure disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, 10% soil temperature 80 respectively, add...

Embodiment 3

[0084] Oral mucosal exudate colloidal gold method for detecting HIV antibody sample diluent, including the following components:

[0085] Raw material name Standard concentration ( / L)

[0086] Disodium hydrogen phosphate 18.5g

[0087] Sodium dihydrogen phosphate 2.8g

[0088] Sodium chloride 8.5g

[0089] 10% Tween 80 10.0ml

[0090] Chicken Serum 145.3ml

[0091] Avidin 30.2mg

[0092] Biotin 20ml

[0093] EDTA-2Na 20g

[0094] NaN3 3.0ml

[0095] The balance is purified water

[0096] The preparation method of the sample diluent for detecting HIV antibody by the colloidal gold method of the oral mucosa exudate comprises the following steps:

[0097] A Measure purified water in a stirrer beaker, and start stirring, wherein, the volume of purified water measured accounts for 0.6 times of the total volume of the diluent fixed solution;

[0098] B Measure disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, and 10% soil temperature 80 respectivel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sample diluent of oral mucosal transudate for detecting HIV antibody by colloidal gold method, and a preparation method thereof, belonging to the technical field of biological detection. The diluent comprises disodium hydrogen phosphate 17.3-19.2 g / L, sodium dihydrogen phosphate 2.6-2.9 g / L, sodium chloride 8.3-9.2 g / L, 10% tween 80 9.5-10.5 mL / L, serum 142.5-157.5 mL / L, streptavidin 28.5-31.5 mg / L, biotin 19-21 mL / L, EDTA-2Na 19-21 g / L, NaN3 2.9-3.2 mL / L, and the balance of purified water. The inventive sample diluent can accelerate reaction speed and enable detection to be smoother.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a sample diluent for detecting HIV antibody by the colloidal gold method of oral mucosa exudate and a preparation method thereof. Background technique [0002] Immunocolloidal gold technology is a simple, fast and accurate detection technology developed in the 1990s. Its principle is similar to double-sandwich ELISA. All reagents were immobilized on a microporous nitrocellulose membrane in advance. When using, place one end in the detection solution, and the nitrocellulose membrane can be passed through by the liquid, if the detection solution contains relevant antigens or antibodies. A light red line will appear in the detection area, which means it is positive, otherwise it is negative. Since the technology came out, it has been continuously adopted clinically. This method does not rely on sophisticated instruments, does not rely on skilled technicians, and has simple oper...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
Inventor 喻洪跃竺诚吴纬戴琪珩杨显福肖玲孙小成张容
Owner CHENGDU XIEHE BIOLOGICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com