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Endosulfan degradation stain and application thereof in soil remediation

A technology for degrading bacteria and endosulfan, applied in the field of biological treatment of environmental pollutants, can solve the problems of long residual period, inability to completely degrade endosulfan, high toxicity, etc. The effect of convenient fermentation production

Inactive Publication Date: 2012-10-31
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is that the above-mentioned existing endosulfan microorganisms cannot fully degrade 100 mg / L endosulfan within 10 days, and The degradation products are mostly endosulfan sulfate, which is more toxic, has a longer residual period and is more harmful to the environment, and provides a high-efficiency endosulfan-degrading bacterium

Method used

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  • Endosulfan degradation stain and application thereof in soil remediation
  • Endosulfan degradation stain and application thereof in soil remediation
  • Endosulfan degradation stain and application thereof in soil remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Stenotrophomonas rhizophila Separation of LD-6 and Degradation Ability of Endosulfan

[0030] The sludge near the sewage outlet of Zhejiang Tonglu Pesticide Factory was collected on site, packed in a 150 mL triangular flask, and quickly brought back to the laboratory. The retrieved samples were placed in endosulfan selective basal inorganic salt liquid medium for acclimation culture. The medium composition is (g / L): MgCl 2 0.2, NH 4 NO 3 1, KH 2 PO 4 2,K 2 HPO 4 7.5, NaCl 1, endosulfan 0.1, deionized water 1 L, pH 6.8, sterilized by moist heat at 115°C for 30 minutes before use. The culture conditions are: 28°C, 120 r / min shaking culture, and the liquid volume in the container is 1 / 3 of the container volume. Thereafter, the shaker flask was kept still for a while every 6-7 days, the supernatant was discarded, and then an equal amount of freshly configured selective liquid medium was added to continue the shaking enrichment culture. Four...

Embodiment 2

[0033] Example 2: Stenotrophomonas rhizophila LD-6 drug resistance experiment

[0034] Tetracycline and kanamycin were selected for testing by the filter paper method, and the diameter of the inhibition zone of each antibiotic filter paper (30 μg / piece) on the LD-6 plate cultured with degrading bacteria was used as the criterion for determining sensitivity or resistance . The test results showed that the diameters of the inhibition zones were 24 and 16 mm, showing moderate sensitivity (the range of moderate sensitivity was: tetracycline 23-25 ​​mm; kanamycin 14-22 mm).

[0035] This example shows that when the bacteria are released into the natural environment, super bacteria will not be produced or spread among indigenous bacteria due to drug resistance (resistance), which provides a safety guarantee for its future practical application.

Embodiment 3

[0036] Example 3: Stenotrophomonas rhizophila LD-6 heavy metal minimum inhibitory concentration (MIC) test

[0037] The MIC test design was carried out with reference to the research of Filali et al. (Filali B.K., Taoufik J., Zeroual Y., Dzairi F.Z., Talbi M., Blaghen M. Waste water bacterial isolates resistant to heavy metals and antibiotics. Current Microbiology, 2000, 41( 3): 151-156), pick the same size and activated LD-6 single colony in the culture test tube, shake and culture at 30°C, 160 rpm for 48 hours. The MICs were determined according to the growth of the bacteria, and three replicates were set up in each group to test the tolerance of the bacteria to 13 metal ions. The results are shown in Table 1, the strain has multiple tolerance and strong tolerance.

[0038]

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Abstract

The invention discloses an endosulfan degradation stain and application thereof in soil remediation. The endosulfan degradation stain is a strain LD-6 of Stenotrophomonas rhizophila, is preserved in China general microbiological strain preservation administration center On March 2, 2012, and the preservation number is CGMCC No.5839. The endosulfan degradation stain can realize the complete degradation of 100 mg / L of endosulfan within 10 days under an aerobic condition and has low-toxic or non-toxic degradation products and fast environmental degradation; the strain LD-6 is sensitive to common antibiotics, such as tetracycline and kanamycin and tolerant to 13 tested heavy metals and can inhibit the growth of a plurality of phytopathogens; and the endosulfan degradation stain has capability of efficiently degrading the endosulfan in an actual soil environment, can outstandingly shorten the degrading time of the endosulfan and has practical application significance and value.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a bacterium for efficiently degrading endosulfan and its application in soil environmental pollution restoration. Background technique [0002] As an important means of agricultural production, pesticides play an extremely important role in protecting agricultural production, improving comprehensive agricultural production capacity, promoting stable grain production and increasing farmers' income. China is not only a big producer of pesticides, but also a big user of pesticides. The annual usage and average usage are about 1.2 million tons and 2.34 kg / ha respectively, ranking first in the world. However, the utilization rate of pesticides is only 10-20%, and the rest all enter the soil, water and atmosphere, causing serious pollution. According to statistics, there are about 13-16 million hectares of farmland polluted by pesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 虞方伯管莉菠骆林平单胜道刘畅龙珍杨萍
Owner ZHEJIANG FORESTRY UNIVERSITY
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