Analyte detection

An analyte and optical detection technology, which is applied in the field of detection of analytes, can solve the problems of long time to get results, extended analysis and measurement time, etc.

Inactive Publication Date: 2016-01-13
ITI SCOTLAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These factors together add to the longer time to results discussed above as they result in extended analysis and measurement times
[0016] Thus, known EIS methods, especially ...

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Examples

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example 1

[0090] Example 1 - Studying IES Parameters for Multi-Frequency Analysis

[0091] In order to study the optimal parameters to be used in the method of the first aspect of the invention, any EIS device may be employed. However, the electrodes, electrolytes, liquid media, analytes (and probes, if used) that will be involved in the final analysis will typically be employed in order to ensure that these parameters are as close to optimum as possible.

[0092] In this example, probe-target hybridization on a commercial gold IDE from Abtech was studied. Using an electrochemical cleaning cycle, two electrodes in the IDE pair were subjected to 2 SO 4 Linear potential sweep of Ag / AgCl in aqueous solution between -0.6V and +1.65V for 30-40 complete cycles at a sweep rate of 50mV until a stable cyclic voltammogram (CV) of a clean gold electrode is seen feature. Before preparing the DNA (69-merITI021) solution, the DNA probes were passed through the MicroSpin after cleavage of disulf...

example 2

[0111] Example 2 - Studying real-time kinetic measurements using EIS

[0112] In this example, the kinetics of probe-target hybridization on a commercial gold IDE from Alberta Tech was studied. Using an electrochemical cleaning cycle, the two electrodes in the IDE pair were subjected to 2 SO 4 Linear potential sweep between -0.6V and +1.65V of Ag / AgCl in aqueous solution, 30-40 full cycles at a sweep rate of 50mV until a stable cyclic voltammogram (CV) of a clean gold electrode is seen feature. Before preparing this DNA (69-merITI021) solution, pass the DNA probe through the MicroSpin after cleavage of the disulfide-protected nucleotide with 5 mM TCEP solution TM G-25 column (Amersham Biosciences (Amersham Biosciences), Buckinghamshire, UK) was used for purification.

[0113] Immediately after cleaning, the thiol-DNA probe layer was submerged in 2xSSC buffer and (Fe(CN) 6 ) 3- with (Fe(CN) 6 ) 4- Each l0mM (10mM (Fe(CN) 6 ) 3- / 4- ) in 10 μM DNA solution. EIS mea...

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Abstract

Provided is a method for detecting an analyte, which method comprises: a) applying an alternating voltage to the analyte, wherein the alternating voltage comprises a plurality of superimposed frequencies sufficient to distinguish the presence of the analyte by electrochemical impedance spectrometry (EIS); and b) determining the identity and/or quantity of the analyte from EIS data.

Description

technical field [0001] The present invention relates to methods of detecting an analyte using enhanced electrochemical impedance spectroscopy (EIS) techniques to obtain data on the analyte. This approach is advantageous because it can yield increased speed over known EIS assays, and thus can improve time to result (TTR) and facilitate the development of such assays in a near-patient setting. Background technique [0002] Methods for detecting analytes are well known in the field of biochemical analysis. In conventional methods the analyte is typically labeled with a detectable (eg, by fluorescence detection) fluorescent label to allow identification of the analyte. [0003] In the past few years, nanoparticles have been used as markers in the field of DNA detection. These markers are potentially useful in any system that allows labeling and involves binding interactions and thus may be useful in living cell systems as well as proteins and nucleic acids. These nanoparticle...

Claims

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Application Information

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IPC IPC(8): G01N27/02G01N33/487G01N33/543C12Q1/68
CPCC12Q1/6883C12Q2600/118G01N27/3277G01N33/54373G01N33/582G01N33/585G01N27/026
Inventor 安迪·芒特米赞·孔得克尔伊莱尼亚·恰尼蒂尔·巴赫曼彼得·迦扎尔
Owner ITI SCOTLAND
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