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Process for preparing ademetionine butanedisulfonate

A technology of adenosylmethionine butanedisulfonate and adenosylmethionine, which is applied in the field of preparation of adenosylmethionine butanedisulfonate, can solve the problems of long fermentation period, cumbersome steps and the like, and achieves short fermentation period, high product purity, separation of good effect

Active Publication Date: 2012-10-24
WUXI FORTUNE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of adenosylmethionine succinate through microbial fermentation is still the main way of industrial production, but the current process has a long fermentation cycle and cumbersome steps

Method used

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  • Process for preparing ademetionine butanedisulfonate
  • Process for preparing ademetionine butanedisulfonate
  • Process for preparing ademetionine butanedisulfonate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Prepare inclined plane

[0027] First weigh 20g of agar into an enamel cup, add an appropriate amount of distilled water and boil to dissolve it. Then weigh 20g of glucose, 5g of yeast extract and 1g of sodium chloride into a small beaker, add a small amount of water and stir to dissolve, then pour into an enamel cup and mix well, and finally set the volume to 1L, and adjust the pH to 7.0, put it into a 20×200 test tube, and the sample volume is 20ml. Put a cotton plug on it, put it on a slant, and get the slant culture medium after cooling.

[0028] 2. Preparation of inclined spores

[0029] Insert the strain Saccharomyces cerevisiae ATCC11909 into the plate medium, culture at 26°C, and after 6 days, move the single colony to the slant medium obtained in step 1, cultivate at 26°C, for 6 days, to obtain slant spores, and culture on the plate The basic formula is 2.0% by weight of glucose, 0.5% by weight of yeast extract, 0.1% by weight of sodium chloride, 2.0% by ...

Embodiment 2

[0043] 1. Prepare inclined plane

[0044] First weigh 20g of agar into an enamel cup, add an appropriate amount of distilled water and boil to dissolve it. Then weigh 20g of glucose, 5g of yeast extract and 1g of sodium chloride into a small beaker, add a small amount of water and stir to dissolve, then pour into an enamel cup and mix well, and finally set the volume to 1L, and adjust the pH to 7.0, divided into 20×200 test tubes, the loading volume is 20ml. Put a cotton plug on it, put it on a slant, and get the slant culture medium after cooling.

[0045] 2. Preparation of inclined spores

[0046] Digging and inserting the strain Saccharomyces cerevisiae ATCC11909 into the plate culture medium, cultured at 28°C, and after 8 days, moved the single colony to the slant medium obtained in step 1, cultured at 28°C, for 8 days, to obtain slant spores. The plate medium formula is 2.0% by weight of glucose, 0.5% by weight of yeast extract, 0.1% by weight of sodium chloride, 2.0% ...

Embodiment 3

[0061] 1. Prepare inclined plane

[0062] First weigh 20g of agar into an enamel cup, add an appropriate amount of distilled water and boil to dissolve it. Then weigh 20g of glucose, 5g of yeast extract and 1g of sodium chloride into a small beaker, add a small amount of water and stir to dissolve, then pour into an enamel cup and mix well, and finally set the volume to 1L, and adjust the pH to 7.0, put it into a 20×200 test tube, and the sample volume is 20ml. Put a cotton plug on it, put it on a slant, and get the slant culture medium after cooling.

[0063] 2. Preparation of inclined spores

[0064] Dig and insert the strain Saccharomyces cerevisiae ATCC11909 into the plate culture medium, cultivate at 30°C, and after 7 days, move the single colony to the slant medium obtained in step 1, cultivate at 30°C, for 7 days, to obtain slant spores. The plate medium formula is 2.0% by weight of glucose, 0.5% by weight of yeast extract, 0.1% by weight of sodium chloride, 2.0% by ...

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Abstract

The invention discloses a process for preparing ademetionine butanedisulfonate. The process comprises the steps of conducting seed culture, fermentation and refinement sequentially on bacterial strain saccharomyces cerevisiae and then obtaining finished products of the ademetionine butanedisulfonate. The process has the advantages of being short in fermentation period, good in separation effect and high in product purity.

Description

technical field [0001] The invention relates to a preparation method of adenosylmethionine succinate. Background technique [0002] Some microorganisms can accumulate adenosylmethionine succinate in the cells. Through these microorganisms, especially adding a certain amount of precursor substance L-methionine to the medium, the synthesis ability of the bacteria is greatly enhanced. At present, the extraction of adenosylmethionine succinate through microbial fermentation is still the main way of industrial production, but the current process has a long fermentation cycle and cumbersome steps. Contents of the invention [0003] The object of the present invention is to provide a kind of preparation method of adenosylmethionine succinate. [0004] According to one aspect of the present invention, the preparation method of adenosylmethionine succinate is provided: [0005] Saccharomyces cerevisiae is activated, seed cultured, fermented and refined in order to obtain the fini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C07H19/167C07H1/06C12R1/865
Inventor 史佳栋付静朱培硕张莹徐建国
Owner WUXI FORTUNE PHARMA
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