Microbial foliage fertilizer, and preparation method and application thereof
A microbial and foliar fertilizer technology, applied in application, fertilization device, fertilizer mixture, etc., can solve the problems of yield, quality, disease resistance and pest resistance that cannot meet the demand, few types of bacteria, and insufficient nutrition.
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[0021] The preparation method of above-mentioned microbial foliage fertilizer, comprises the steps:
[0022] (1) Inoculate Bacillus subtilis ACCC03120, Bacillus jelly-like CICC23640, Bacillus megaterium CICC21694, and Bacillus thuringiensis CICC20565 respectively in liquid seed medium under aseptic conditions, and culture them at 30-37°C for 18-24 hours, Then inoculate in a liquid fermentation medium with an inoculation amount of 10-20 wt%, and cultivate at 30-37° C. for 3-5 days;
[0023] Prepared respectively containing Bacillus subtilis ACCC03120 viable count 3~8×10 8 Bacteria agent A of each / mL, containing jelly-like bacillus CICC23640 viable count 3~8×10 8 Bacteria agent B of each / mL, containing Bacillus megaterium CICC21694 live bacteria number 1~5×10 8 Each / mL of bacterial agent C, containing Bacillus thuringiensis CICC20565, the number of viable bacteria is 3~8×10 8 Individual / mL of bacterial agent D;
[0024] (2) Inoculate Azotobacter pheoglobosa ACCC11104 in liqu...
Embodiment 1
[0060] A preparation method of microbial leaf painting fertilizer, comprising the steps of:
[0061] (1) Inoculate Bacillus subtilis ACCC03120, Bacillus jelly-like CICC23640, Bacillus megaterium CICC21694, and Bacillus thuringiensis CICC20565 respectively in liquid seed medium under aseptic conditions, cultivate them at 30°C for 24 hours, and then press 10wt% The inoculum amount was inoculated in the liquid fermentation medium and cultivated at 30°C for 3 days;
[0062] The number of live bacteria containing Bacillus subtilis ACCC03120 was 8×10 respectively. 8 Bacteria agent A of each / mL, containing jelly-like Bacillus CICC23640, the number of viable bacteria is 6×10 8 Bacteria agent B containing Bacillus megaterium CICC21694 count 4×10 per mL 8 Bacillus thuringiensis CICC20565 live bacteria count 6×10 of bacterial agent C of each / mL 8 Individual / mL of bacterial agent D;
[0063] The seed culture medium in the described step (1) is prepared by mixing and sterilizing the fo...
Embodiment 2
[0086] The microbial foliar fertilizer obtained by the preparation method as described in Example 1, the difference is that in step (4), the prepared bacterial agent A, bacterial agent B, bacterial agent C, bacterial agent D, bacterial agent E and bacterial agent F are mixed according to the ratio of volume ratio 1:1:2:2:2:2, and the composite microbial bacterial agent is prepared, and the total number of bacteria is 5×10 8 individual / mL.
[0087] Among them: the number of live bacteria of Bacillus subtilis (Bacillus subtilis) ACCC03120 8×10 7 Individual / mL, Bacillus mucilaginosus (Bacillus mucilaginosus) CICC23640 viable count 6×10 7 Individual / mL, Bacillus megaterium (Bacillus megaterium) CICC21694 viable count 8×10 7 Individual / mL, Bacillus thuringiensis (Bacillus thuringiensis) CICC20565 viable count 12x10 7 Individual / mL, Azotobacter chroococcum (Azotobacter chroococcum) ACCC11104 live bacteria count 10×10 7 Individual / mL, Saccharomyces cerevisiae (Saccharomyces cerev...
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