A kind of harm reduction additive for tobacco and its preparation method and application
A technology for additives and cigarettes, applied in the application, preparation of tobacco, tobacco, etc., to achieve the effect of clear biological harm reduction
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[0028] Cytotoxicity Evaluation of Natural Plant Tobacco Harm Reduction Additives Added to Tobacco Smoke
[0029] 1 Purpose of the test
[0030] To evaluate the cytotoxicity of cigarettes with different treatments.
[0031] 2 test items
[0032] There are 7 kinds of cigarettes, labeled as control cigarettes and experimental cigarettes (natural tobacco with harm-reducing additives, the addition ratios are 0.02%, 0.05%, 0.1%, 0.2%, 0.5%, and 1%).
[0033] 3 test plan
[0034] 3.1 Preparation of Tobacco Condensate (CSC)
[0035] Collect cigarette smoke according to standard smoking conditions (GB / T16450-1996, standard conditions defined by smoking machines for routine analysis), prepare smoke condensate (CSC), and prepare a solution of 1 cigarette / ml with cell culture medium, - Store at 80°C for later use. When experimenting, dilute to the experimental design concentration.
[0036] 3.2 Cell culture
[0037] Beas-2B cells were cultured in serum-free LHC-8 medium at 37°C, 5%...
Embodiment 1
[0047] Take Epimedium, Eclipta, sweet almond, motherwort, dandelion and Huajuhong respectively, grind them and mix them uniformly according to the ratio of 0.5:0.5:0.2:0.3:0.3:1, add 8 times the amount of pure water, soak for 2 hours, 75 Heat reflux at ℃ for 1 hour, extract 3 times in total, combine the extracts from the 3 times, concentrate at 80 ℃ and pressure 0.02Mpa to a fluid extract with a density of 1.12. Add 2 times the volume of ethanol, let it stand for 24 hours, filter the precipitate, concentrate to a density of 1.12 liquid extract under the conditions of 80°C and a pressure of 0.02Mpa, and filter to obtain the harm-reducing additive for tobacco. Take 10g of the additive, dissolve it in 20kg of distilled water, spray it on 500kg of shredded tobacco, and roll it into cigarettes.
Embodiment 2
[0049] Take Epimedium, Eclipta, Sweet Almond, Motherwort, Dandelion, and Juhong respectively, grind them and mix them evenly at a ratio of 1:0.8:0.5:0.5:0.5:1, add 6 times the amount of pure water, soak for 6 hours, and 70 ℃ heat reflux extraction for 1.5h, a total of 3 extractions, combined 3 extracts, 75 ℃, concentrated under the conditions of pressure 0.06Mpa to a density of 1.11 liquid extract. Add 2 times the volume of ethanol, let it stand for 36 hours, filter the precipitate, concentrate at 75°C and a pressure of 0.06Mpa to a liquid extract with a density of 1.11, and filter to obtain the harm-reducing additive for tobacco. Take 50g of the additive, dissolve it in 20kg of distilled water, spray it on 500kg of shredded tobacco, and roll it into cigarettes.
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Abstract
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Application Information
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