Microbial preparation used for improving penaeus vannemei immunity
A microbial preparation and immunity technology, applied in the field of microbial preparations, can solve the problems of deterioration of aquaculture environment, loss of aquaculture, decline of shrimp physique, etc., and achieve the effect of promoting phagocytic activity, high content of viable bacteria, and recovery of gastrointestinal function.
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Embodiment 1
[0024] First weigh 3 parts of phosphate, 15 parts of glucose, and 1 part of nitrogen beef extract powder to make fermentation auxiliary materials.
[0025] Then weigh 2 parts of Bacillus licheniformis, 3 parts of Bacillus subtilis and 1 part of the above-mentioned fermentation auxiliary material, mix evenly, place in a fermenter, and ferment at 30°C for 5 days to obtain a microbial preparation. The number of live bacteria of the bacteria preparation obtained by detection was Bacillus licheniformis 10 8 Individual / g, Bacillus subtilis 10 5 pcs / g.
[0026] Both the Bacillus subtilis and the Bacillus licheniformis were purchased from China Industrial Microorganism Culture Collection Management Center, specifically Bacillus subtilis CICC 10090 and Bacillus licheniformis CICC20205.
Embodiment 2
[0028] First weigh 1 part of phosphate, 20 parts of glucose, and 2 parts of nitrogen beef extract powder to make fermentation auxiliary materials.
[0029] Then weigh 2 parts of Bacillus licheniformis, 8 parts of Bacillus subtilis and 1 part of the above-mentioned fermentation auxiliary material, mix them evenly, place them in a fermenter, and ferment at 30° C. for 5 days to obtain a microbial preparation. The number of viable bacteria in the detected bacterial preparation was Bacillus subtilis 10 9 Individual / g, Bacillus licheniformis 10 6 pcs / g.
Embodiment 3
[0031] First weigh 2 parts of phosphate, 25 parts of glucose, and 1 part of nitrogen beef extract powder to make fermentation auxiliary materials.
[0032] Then weigh 2 parts of Bacillus licheniformis, 6 parts of Bacillus subtilis and 1 part of the above-mentioned fermentation auxiliary material, mix evenly, place in a fermenter, and ferment at 30° C. for 5 days to obtain a bacterial preparation. The number of viable bacteria in the detected bacterial preparation was Bacillus subtilis 10 8 Individual / g, Bacillus licheniformis 10 5 pcs / g.
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