Method for culturing and rapidly propagating large-flowered cymbidiums

A technology for orchids and large flowers, which is applied in the field of breeding and rapid propagation of new varieties of large-flowered orchids, and can solve the problems of hybrid breeding and rapid propagation of large-flowered orchids that have not been reported.

Inactive Publication Date: 2012-10-17
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although someone has done research on breeding and seed germination of orchids, there are no reports on the hybrid breeding and rapid propagation of large-flowered orchids.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Breeding and propagation of BCL-1 (Bichunlan)

[0030] 1. Source of material: two parent orchid strains Jasperopsis ( Cmbidium lowianum) and exclusive spring ( C. eburneum ) is collected from the mountainous area of ​​Yunnan, and Jasper Magnolia and Duozhanchun are the parents of each other.

[0031] 2. Training method

[0032] (1) Hybrid breeding

[0033] ① Establish an orchid resource garden. The environment in the resource garden can be manually regulated to create the best environment for the growth and development of orchids.

[0034] ② The natural flowering periods of these orchids are close to the same, and the breeding operation will be carried out when 1 / 2 of the inflorescence buds of the parents are open.

[0035] ③ Breeding operation: Clean your hands and tweezers, gently remove the parent herb cap (anther top cap) with your hands, use tweezers to pick off the pollen block of the male parent, and put it into the cavity (stigma) of the female ...

Embodiment 2

[0046] Embodiment 2: the breeding and propagation of BHL-1 (bicymbidium)

[0047] Female parent: Jasper ( C. lowianum ) ； Male parent: Cymbidium ( C. hyridus )

[0048] (1) Hybrid breeding is the same as in Example 1.

[0049] (2) Aseptic germination of seeds

[0050] ① Seed germination medium: The improved MS (i.e. 1 / 2MS) is selected as a more suitable basic medium, and the formula with growth regulator is: improved MS+6-BA2.0mg.L -1 +NAA0.5mg.L -1 . Culture conditions: the same as in Example 1.

[0051] ②After the seeds germinate, sprouts first, and then radicles grow, then transfer to the improved MS+6-BA0.3mg.L -1 +NAA0.5mg.L -1 Cultured in the culture medium, after 4 to 6 months, it gradually becomes a test-tube plantlet with a root system and can be hardened and transplanted.

[0052] (3) Proliferation, differentiation induction and rooting culture

[0053] ① Protocorm Proliferation Medium: Improved MS + 6-BA 2.0mg.L -1 + NAA 0.5mg.L -1 , cultivated for ...

Embodiment 3

[0058] Example 3: Breeding and Propagation of WHL-1 (Wilden Tiger Orchid)

[0059] Female parent: Wenshan Hongzhulan ( C. wenshanense ); male parent: Tiger Xuelan ( C. tracyanum var huanghua× C. mastersii ), hybrid breeding and seed aseptic germination are the same as in Example 1 or Example 2.

[0060] Proliferation, differentiation induction and rooting culture:

[0061] ① Protocorm Proliferation Medium: Improved MS + 6-BA3.0mg.L -1 + NAA 0.8mg.L -1 , cultivated for 42 days.

[0062] ② Protocorm differentiation into test tube seedling medium: improved MS + 6-BA2.5mg.L -1 + NAA 0.8mg.L -1 + Banana puree 80g / L, cultivated for 53 days.

[0063] ③ Rooting medium: improved MS + 6-BA0.8mg.L -1 + NAA 2.5mg.L -1 , cultivated for 36 days.

[0064] Proliferation, differentiation induction and rooting culture conditions were the same as in Example 1.

[0065]

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PUM

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Abstract

The invention discloses a method for culturing and rapidly propagating large-flowered cymbidium. According to the invention, crossbreeding and fruit culture are carried out on orchid plants such as cymbidium lowianum, cymbidium eburneum, cymbidium wenshanense, cymbidium hybridium and the like in a resource garden; when the fruits of the cymbidiums are cultured to 90% mature and the fruit peels slightly become yellow, the fruits are picked for crossbreeding; explant treatment and seed sterile germination culture are carried out to ensure that the seeds germinate to root; transiting the root into a modified culture medium for culture; after 4-6 months, the seeds gradually become test-tube plantlets which have root systems and can be exercised and transplanted; through multiplication, induction and rooting culture, the test-tube plantlets can be taken out for exercise when the roots are about 1cm long; and after 1-2 months, culture is carried out in a greenhouse by using facilities. The method provided by the invention is not only capable of culturing new species, but also capable of carrying out rapid propagation, so as to satisfy the industrialized demands of fragrant flowers and bright colors.

Description

[0001] technical field [0002] The invention relates to the technical field of plant cultivation, in particular to a method for breeding and rapid propagation of new varieties of large-flowered orchids. Background technique [0003] Large-flowered orchids refer to the genus Orchids ( Cmbidium ) in the large flower subgenus, the main feature is the large flower type. The flowering period of orchids is different. It is necessary to control the flowering period or save pollen to pollinate on time. Orchid hybrid seeds are small, contain some immature embryos, no endosperm, and it is difficult to germinate in a natural state. Although there has been research on the breeding and seed germination of orchids, there are no reports on the hybrid breeding and rapid propagation of large-flowered orchids. Contents of the invention [0004] The purpose of the present invention is to provide a method for breeding and rapid propagation of large-flowered orchids that lays an importa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 李枝林杨根华王有国唐敏张小平张爱玲李叶芳
Owner YUNNAN AGRICULTURAL UNIVERSITY
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