Control system for DNA (Deoxyribose Nucleic Acid) sequencer

Abstract

The invention discloses a control system for a DNA (Deoxyribose Nucleic Acid) sequencer, which comprises a PLC (Programmable Logic Controller), controllers of first and second servo motors, first and second peristaltic pumps and driving motors of first and second multipass reversing valves, wherein the controllers of the first and second servo motors are electrically connected with the PLC; the first and second peristaltic pumps are respectively and electrically connected with the PLC and are used for receiving control signals of the PLC; a CCD (Charge Coupled Device) camera is electrically connected with the PLC and is used for receiving a control signal of the PLC; and a plurality of sensors are respectively and electrically connected with the PLC and are used for sending position signals of the CCD camera to the PLC. According to the control system disclosed by the invention, a reagent supply assembly of the DNA sequencer with a plurality of reaction bins timely and accurately supplies reagents and buffer solution for a plurality of reaction bins and the CCD camera can be ensured to timely read an optical signal in each reaction bin, and therefore, the aim of simultaneously performing a plurality of reactions is fulfilled, so that a plurality of samples can be simultaneously sequenced and the DNA sequencing efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing, in particular to a control system for a DNA sequencer. Background technique [0002] In the field of DNA sequencing technology, pyrosequencing technology (pyrosequencing) is a new type of enzymatic cascade sequencing technology developed by Nyren et al. in 1987. Its repeatability and accuracy performance are comparable to Sanger method DNA sequencing technology. , while the speed is greatly improved. [0003] Pyrosequencing technology is an enzyme cascade chemiluminescent reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primer anneals to the template DNA, the four enzymes DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate (Apyrase) Under the synergistic effect of the primer, the polymerization of each dNTP on the primer is coupled with the release of...

AI Technical Summary

Problems solved by technology

[0007] The inventor found that the DNA sequencer in the prior art has only one reaction chamber, and one instrument can only perform one test, and the work efficiency is not high

In order to improve work efficiency, the inventor has produced a DNA sequencer with multiple reaction chambers. Due to the existence of multiple reaction chambers, there are timely supply of reaction reagents and buffers for multiple reaction chambers and the use of a CCD camera to timely collect multiple Coordination of Optical Signals in Reaction Chambers

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