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Mesoporous biomaterial containing porcine pancreatic lipase and preparation method of mesoporous biomaterial

A technology of biological materials and mesoporous materials, which is applied in the same direction fixed on or in an inorganic carrier to achieve the effect of large pore size, large specific surface area and environmental friendliness

Inactive Publication Date: 2012-09-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study of this composite porcine pancreatic lipase functionalized mesoporous material has not been reported yet, and it can be expected that such a composite functional material will not only be used in the field of biocatalysis, but also in the field of nano-bioluminescent sensor devices, biomolecules, etc. Carriers and other aspects have broad application prospects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve 2 grams of non-ionic surfactant P123 in 15 grams of water and 60 grams of 2M hydrochloric acid solution, stir at room temperature for 30 minutes until clear, place it in a water bath at 40°C, add tetraethyl orthosilicate 3.83 grams, after pre-hydrolysis for 2 hours, add 0.49 grams of chloropropyl-triethoxysilylating reagent, stir at 40°C for 24 hours, then transfer to a reaction kettle with a polytetrafluoroethylene liner, and place it at 100°C In an oven, aged for 24 hours, filtered, washed, and dried naturally in the air, and finally extracted in ethanol hydrochloric acid solution for 24 hours to remove the surfactant, dried at room temperature, and the proportion of chlorine groups in the total silicon was 10. % mesoporous silica (SBA-Cl-10%). The above materials were added to 20ml of porcine pancreatic lipase buffer solution with a concentration of 2mg / mL, and the mixture was mixed at 25°C and 215rmp for 3 hours. Then, place it at 10° C. and centrifuge at ...

Embodiment 2

[0027] Dissolve 4 grams of nonionic surfactant P123 in 125 grams of 1M hydrochloric acid solution, stir at room temperature for 35 minutes until clear, place it in a water bath at 35°C, add 4.04 grams of tetraethyl orthosilicate, and pre-hydrolyze 1.5 Hours later, add 0.25 g of mercaptopropyl-triethoxysilylating reagent, stir at 35°C for 24 hours, then transfer to a reaction kettle with a polytetrafluoroethylene liner, put it in a 100°C oven, and age for 24 hours. Hours, filtered, washed, dried naturally in the air, and finally extracted in ethanol hydrochloric acid solution for 24 hours to remove the surfactant, dried at room temperature to obtain a mesoporous silica containing mercapto groups in the total silicon with a ratio of 5%. (SBA-SH-5%). The above materials were added to 20ml of porcine pancreatic lipase buffer solution with a concentration of 2mg / mL, and the mixture was mixed for 1 hour at 25°C and 215rmp. Then, place it at 10° C. and centrifuge at 6000 rpm for 10 ...

Embodiment 3

[0029] Dissolve 1 gram of non-ionic surfactant P123 in 7.5 grams of water and 30 grams of 4M hydrochloric acid solution, stir at room temperature for 40 minutes until clear, place it in a water bath at 45°C, add 1.4 methyl orthosilicate grams, after pre-hydrolysis for 1 hour, add 0.20 grams of vinyl-triethoxy silylating reagent, stir at 45°C for 24 hours, then transfer to a reaction kettle with a Teflon liner, and put it into a 100°C oven , aged for 24 hours, filtered, washed, and dried naturally in the air, and finally extracted in ethanol hydrochloric acid solution for 24 hours to remove the surfactant, dried at room temperature, and the proportion of vinyl groups in the total silicon was 10%. Mesoporous silica (SBA-YX-10%). The above materials were added to 20ml of porcine pancreatic lipase buffer solution with a concentration of 2mg / mL, and the mixture was mixed at 25°C and 215rmp for 9 hours. Then, place it at 10° C. and centrifuge at 6000 rpm for 10 minutes, filter off ...

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PUM

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Abstract

The invention discloses a mesoporous biomaterial containing porcine pancreatic lipase and a preparation method of the mesoporous biomaterial. The method comprises the steps of dissolving surfactant in water solution of hydrochloric acid, adding inorganic silicon source and organic silylating reagent, fully hydrolyzing under a water bath condition, aging, cooling, filtering, washing, drying and extracting the surfactant to obtain mesoporous silica containing an active group, then putting the prepared material into buffer solution of porcine pancreatic lipase, centrifuging, filtering and drying to obtain the mesoporous biomaterial with functionalized porcine pancreatic lipase. The method disclosed by the invention is simple and environment-friendly. The synthetic mesoporous biomaterial containing the porcine pancreatic lipase has the characteristics of large aperture, high specific surface area, high enzyme activity and large enzyme activity increasing proportion, and can be directly used as biological catalytic material. The mesoporous biomaterial containing the porcine pancreatic lipase disclosed by the invention has wide application prospect in these respects of not only bioreactors, but also nano-bio-sensors, biomolecular delivery and the like.

Description

technical field [0001] The invention relates to a mesoporous biological material containing porcine pancreatic lipase and a preparation method thereof, in particular to a mesoporous silicon oxide material functionalized with porcine pancreatic lipase in pores and a preparation method thereof. Background technique [0002] In recent years, the international attention-grabbing research hotspot is the interdisciplinary subject of inorganic materials and life sciences. Various biomolecules are encapsulated into the cavity of porous inorganic materials through specific physical or chemical methods, resulting in a class of The advent of amazing new bio-inorganic hybrid materials, using inorganic materials as carriers to encapsulate lipase, DNA, insulin, amino acids, cytochrome C, etc. has been widely studied. Among them, the composite functional materials obtained by effectively immobilizing enzymes play an important role in different fields such as bioanalysis, biocatalysis, prot...

Claims

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Application Information

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IPC IPC(8): C12N11/14
Inventor 王建强郭成朱凯杜仁军王伟栾长军唐拾贵
Owner NANJING UNIV OF TECH
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