Multiplying culture media for strawberry

Abstract

The invention discloses a multiplying culture media for strawberry, comprising 1 / 2 improved MS, 0.2-0.5 mg / L 6-BA, 100-200 mg / L of glutamine, 30-50 g / L of white sugar, and 0.35% of phytagel, wherein the pH value ranges from 5.5 to 6.0. By selecting the improved MS as a basal medium of strawberry culture media, the content of the organic matter of the culture media provided by the invention is greatly increased; with the replacement of agar by using the phytagel, the inherent defect of the traditional agar in the plant tissue culture can be effectively overcome, the genotype limitation problemof strawberry high-frequency embryogenesis in regeneration culture can be overcome, the plant regeneration frequency and the inducement frequency of somatic embryos can be greatly increased, and the breeding efficiency can be improved.

Description

technical field [0001] The invention relates to a herbaceous plant culture medium, in particular to a strawberry value-added culture medium, which belongs to the technical field of plant biology. Background technique [0002] Strawberry has been promoted and planted in a large area in my country because of its outstanding economic value and ecological function. In the face of production needs and existing production conditions, strawberry needs to be further improved in terms of yield, quality and resistance. Combining biotechnology and conventional breeding methods to breed new strawberry varieties is an effective way to improve the efficiency of strawberry breeding. [0003] The traditional strawberry proliferation medium is: MSM basic medium + 0.1-0.5mg / L 6-BA + 100-200mg / L glutamine + 20-30 g / L sugar + 5-9g / L agar, the pH value is 5.5~6.0. First of all, in terms of composition, the content of organic matter in the MSM basic medium is relatively low, and does not cont...

AI Technical Summary

Problems solved by technology

First of all, in terms of composition, the content of organic matter in the MSM basic medium is relatively low, and does not contain the component inositol, which is not conducive to the growth and development of plants. Secondly, the component agar has insurmountable inherent defects in plant tissue culture, which affects The overall effect of the medium