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Germination method of Leymus chinensis seeds

A technology of Leymus chinensis and seeds, applied in the directions of seed and rhizome treatment, application, agriculture, etc., can solve the problems of strong dormancy of Leymus chinensis seeds, slow germination of Leymus chinensis, inconsistent maturity, etc. Simple and easy method

Inactive Publication Date: 2012-09-19
HEILONGJIANG HEIKE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Leymus chinensis seeds have strong dormancy and inconsistent maturity, resulting in slow germination and low emergence rate of Leymus chinensis

Method used

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  • Germination method of Leymus chinensis seeds
  • Germination method of Leymus chinensis seeds
  • Germination method of Leymus chinensis seeds

Examples

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specific Embodiment approach 1

[0014] Specific embodiment one: the present embodiment Leymus chinensis seed germination method is as follows:

[0015] 1. Soak the lemma-removed Leymus chinensis seeds in sterile water at 5°C and protected from light for 0, 2, 4, 6 or 8 days;

[0016] 2. Soak Leymus chinensis seeds treated in step 1 with ethanol with a volume concentration of 75% for 30 seconds, rinse with sterile water for 3 times, disinfect with NaClO solution with a volume concentration of 10% for 8 minutes, and rinse with sterile water for 5 to 6 minutes. Second, sow treated Leymus chinensis seeds in MS medium;

[0017] 3. Under the condition of avoiding light, culture at 28°C for 16h, and then at 22°C for 8h, 24h is a cycle;

[0018] 4. Germinate for 2-14 days under the conditions of step 3 to obtain germinated Leymus chinensis seeds.

[0019] The method of the present invention is simple and easy to operate, simple in operation and obvious in effect, and can increase the germination rate of Leymus chi...

specific Embodiment approach 2

[0020] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the Leymus chinensis seeds without lemma described in step one are the Leymus chinensis seeds from which all the palea pieces have been removed. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0021] Embodiment 3: This embodiment is different from Embodiment 1 or 2 in that the Leymus chinensis seeds described in step 1 are the seeds of Leymus chinensis. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a germination method of Leymus chinensis seeds, relating to a seed germination method, and solving the technical problems of long dormancy period and low germination rate of Leymus chinensis seeds. The method comprises the following steps of: 1, soaking shelled Leymus chinensis seeds in sterile water at 5 DEG C and in a dark condition, disinfecting the seeds with ethanol and NaClO solution, washing the seeds with sterile water, and sowing the seeds in an MS culture medium; 2, culturing for 16 hours in the dark condition and at 28 DEG C, then culturing for 8 hours at 22 DEG C, and then circulating by taking 24 hours as a cycle; and 3, germinating for 2-14 days in the conditions in the step 2 to finally obtain the germinated Leymus chinensis seeds. The method disclosed by the invention is simple and easy, simple in operation, obvious in effect, and capable of increasing the germination rate of the shelled Leymus chinensis seeds to 75.42% after low-temperature (5 DEG C) treatment from 54.47% without the low-temperature (5 DEG C) treatment.

Description

technical field [0001] The invention relates to a seed germination method. Background technique [0002] Leymus chinensis (Leymus chinensis) is a perennial herb of the Poaceae Laymus genus. Because of its strong environmental adaptability, cold resistance, drought tolerance, and salt-alkali tolerance, it has become a dominant species in the Songnen Plain. It plays an extremely important role in the control of land desertification in my country. At the same time, Leymus chinensis is also an important forage resource. Leymus chinensis has many leaves, high protein content, rich nutrition, good palatability, and is resistant to trampling. All kinds of livestock like to eat it all year round. It has the reputation of "fine grain for livestock". It has significant economic and social benefits in the development of grassland animal husbandry. The Songnen Plain in northeastern my country and eastern Inner Mongolia are its distribution centers, and it is also distributed in Hebei, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 王雷李瑶
Owner HEILONGJIANG HEIKE TECH CO LTD
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