Processing method of high-theaflavin black tea beverage
A technology of theaflavin black tea and a processing method, which is applied in the processing field of high-theaflavin black tea beverages, can solve the problems of the final black tea beverage flavor, quality and safety, and achieves high health care value, low production cost and reasonable design. Effect
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Embodiment 1
[0023] Example 1: Determination of the activity of polyphenol oxidase and peroxidase in fresh fruit pulp
[0024] The fresh fruit pulp that participates in the fermentation in the present invention mainly refers to the pulp obtained from the mixture of two or more raw materials in apples, pears, peaches, bananas and oranges. The pulp contains polyphenol oxidase (PPO) and peroxidase (POD). The present invention can also use other fresh fruits, fresh tea leaves or other plant materials containing polyphenol oxidase (PPO) and peroxidase (POD). Enzyme activity can be determined as follows.
[0025] Determination of polyphenol oxidase (PPO)
[0026] PPO was determined spectrophotometrically using (+)-catechin as a substrate. The assay was performed in a reaction volume of 3 mL containing 3 mM catechins in 0.1 M phosphate citrate buffer pH 5.5. Utilize a 150 µL aliquot of a freshly prepared 60 mM stock solution of catechin in a water (70% v / v)-ethanol (30% v / v) mixture to obtain...
Embodiment 2
[0029] Embodiment 2: the processing method of high theaflavin black tea beverage
[0030] Take 50 kg of dry green tea (16-40 mesh), add 1000 kg of hot water at 80°C, stir and extract in the extraction tank for 40 minutes, take out the tea soup and filter out the tea dregs, cool the tea soup to 40°C, and pump it into 2m 3 120 kg of apple juice and 80 kg of pear juice prepared in advance were added to the fermentation tank. At this time, the activity of polyphenol oxidase in the fermentation system reached 0.1 u / g min, and the activity of peroxidase reached 0.2 u / g min , after stirring for 5 minutes, adjust the temperature to 37°C, adjust the pH value to 4.5, introduce oxygen and stir for 50 minutes, and the tea juice will turn into black tea juice through oxidation reaction, with a strong fruity and tea aroma, that is, the content of theaflavins When the solid content of the fermented tea soup reaches more than 1%, the reaction is stopped, and the pomace is removed by coarse fi...
Embodiment 3
[0032] Embodiment 3: the processing method of high theaflavin black tea beverage
[0033] Take 50 kg of dry green tea (16-40 mesh), and use 60°C hot water to dynamically extract it in a dynamic countercurrent extraction equipment for 30 minutes at a flow rate of 1500 L / h. ℃, pumped into 2m 3Add 250 kg of pre-prepared apple, pear and orange mixed pulp into the fermenter. At this time, the activity of polyphenol oxidase in the fermentation system reaches 0.36 u / g min, and the activity of peroxidase reaches 0.5 u / g min , after stirring for 5 minutes, adjust the temperature to 30°C, adjust the pH value to 4.0, introduce air and stir for 90 minutes, when the tea juice becomes black tea juice through oxidation reaction, with strong fruity and tea aroma, that is, the content of theaflavins reaches When the solid content of the fermented tea soup is more than 1%, stop the reaction, coarsely filter with a 120-mesh filter to remove pomace, and then separate through a disc centrifuge at...
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