Method for reproducing paphiopedilum armeniacum

A technology of P. chinensis and seeds, which is applied in the field of seed propagation of P. chinensis, and can solve problems such as difficulty in seed germination and the like

Active Publication Date: 2012-08-15
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] The present invention aims at overcoming the above-mentioned problems of prior art, provides a kind of method for seed propagation of Papudendron apricot, utilizes morphological anatomy method and combines seed aseptic germination technology, observes the seed development process of Pachyphytum apricot, analysis germination rate is relatively high. The morphological characterist

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  • Method for reproducing paphiopedilum armeniacum
  • Method for reproducing paphiopedilum armeniacum
  • Method for reproducing paphiopedilum armeniacum

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Embodiment 1

[0016] The method for determining the best germination time of Paphiopedilum apricot seeds, the propagation method of Paphiopedilum apricot:

[0017] 1. Materials and Methods

[0018] 1.1 Research materials

[0019] P. armeniacum S.C.Chen et F.Y.Liu: Distributed in western Yunnan, it grows in limestone wall accumulations or rocky and well-drained grass slopes at an altitude of 1400-2100 meters. With slender and horizontal underground rhizomes, 2-3mm in diameter, 5-7 leaves, pure yellow flowers, scape 15-28cm, deep sac-like lips, nearly elliptical spherical or broad elliptic, flowering period 2 ~April, fruit ripening period is August-September. It was planted in the orchid greenhouse of Kunming Institute of Botany, Chinese Academy of Sciences. The cultivation conditions in the greenhouse are daily temperature 25°C, night temperature 18°C, light intensity 300μmolmP -2 sP -1 , the light time is 12h, and the relative humidity is 60%. Irrigate once every 10 days, and at the s...

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Abstract

The invention relates to a method for reproducing paphiopedilum armeniacum. The method comprises the steps of carrying out field planting on adult plants of the paphiopedilum armeniacum, carrying out artificial pollination when the paphiopedilum armeniacum flowers, marking the time of pollination and seeding by using capsule seeds of the paphiopedilum armeniacum of 90-110 days after the pollination. In recent years, field populations of the paphiopedilum armeniacum are excessively collected, seriously damaged and endangered and problems of protection and commercial development are needed to solve through artificial breeding method. According to the invention, aiming to solve the problem that the paphiopedilum armeniacum seed is more difficult to sprout after maturing, methods of seed morphological characteristic observing and aseptic germination are utilized to confirm that the seed germination rate is highest when the seed blastocyte morphological characteristic is shown as ellipsoidal embryogenesis and the suspensor presents a character of 2-3 cellular components in linear arrangement after 90 days after the pollination of the paphiopedilum armeniacum, therefore, the method disclosed by the invention has the advantages that optimal germination time of the seeds of the paphiopedilum armeniacum can be confirmed and technological basis is established for the building of a high-efficiency artificial reproduction technical system of the paphiopedilum armeniacum.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a seed propagation method of P. armeniacum S.C. Chen et F. Y. Liu of the genus Paphiopedilum. technical background: [0002] Paphiopedilum belongs to the orchid family Cypripedioideae. Currently, there are 79 wild species recorded, about 1 / 3 of which are produced in China. In terms of species, China is the country with the most abundant Paphiopedilum in the world, with a total of 27 species (Liu Zhongjian et al., 2009). One of the notable features of the genus Paphiopedilum is that the lip petals are specialized into pocket-shaped or slipper-shaped, so it is also called "Slipper Orchid" or "Xianlu Orchid" (Luo Yibo et al., 2005). Orchidaceae Paphiopedilum is famous all over the world for its unique flower shape and gorgeous flower color. It is widely concerned and loved by orchid lovers and horticulturalists for its rich flower color and long flowering period. It has gr...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 严宁张娟娟胡虹
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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