On-line sample preparation device for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)
An auxiliary laser and sample preparation technology, applied in the field of mass spectrometry-oriented sample preparation devices, can solve problems such as sample backmixing, zone broadening and cross-contamination, and achieve the effect of retaining resolution, good application prospects, and simplifying the sample preparation process.
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[0025] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.
[0026] see figure 1 , the embodiment of the present invention is provided with a first-stage T-shaped orthogonal structure microfluidic chip, a second-stage T-shaped orthogonal structure microfluidic chip, a matrix reagent injection pump and a target plate 3 .
[0027] The first-stage T-shaped orthogonal structure microfluidic chip is used to prepare the sample separated by liquid chromatography into liquid droplets, and mix the aqueous phase eluent A of liquid chromatography with another immiscible oil Phase B introduces the microfluidic chip of the first-stage T-shaped orthogonal structure at the same time, and the inlet of the vertical arm 11 of the microfluidic chip of the first-stage T-shaped orthogonal structure is connected to the outlet of the aqueous phase eluent of the liquid chromatography; The left cross arm 121 of the first-stage T-shape...
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