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Fast propagation method for cultivating salt-tolerant and low temperature-tolerant periwinkle strain

A periwinkle, low temperature-resistant technology, applied in the field of bioengineering, can solve problems such as unsatisfactory chemical synthesis, and achieve the effect of increasing biomass and prolonging growth period

Inactive Publication Date: 2012-08-01
上海安体康生植物化学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The content of vinblastine and vincristine in plants is extremely small, about 1 / 100,000, and the chemical synthesis is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] This embodiment includes the following steps:

[0017] 1) Preparation of periwinkle growth medium, induction growth medium and salt-tolerant and low-temperature tolerance screening medium: take MS basic medium, add 6 grams of agar powder per liter to obtain periwinkle growth medium for use; take another MS basic medium Culture medium, add 0.1 gram of naphthaleneacetic acid, 3 grams of 6-benzylaminopurine, 0.2 gram of colchicine and 6 grams of agar powder per liter as a unit to obtain an induction growth medium for subsequent use; take another MS basic medium, and use per liter of Add 10 grams of sodium chloride and 6 grams of agar powder as a unit to obtain a salt-tolerant and low-temperature resistant screening medium for future use.

[0018] Adjust the pH value of the growth medium: measure the pH value of the solid growth medium and the liquid growth medium with a pH meter, and adjust the pH value of the periwinkle growth medium, the induction growth medium and the s...

Embodiment 2

[0024] This embodiment includes the following steps.

[0025] 1) Preparation of periwinkle growth medium, induction growth medium and salt-tolerant and low-temperature tolerance screening medium: take MS basic medium, add 6.5 grams of agar powder per liter to obtain periwinkle growth medium for use; take another MS basic medium Culture medium, add 0.15 grams of naphthalene acetic acid, 2 grams of 6-benzylaminopurine, 0.3 grams of colchicine and 6.5 grams of agar powder per liter as a unit to obtain the induced growth medium for subsequent use; another MS basic medium is used for each liter of Add 15 grams of sodium chloride and 6.5 grams of agar powder as a unit to obtain a salt-tolerant and low-temperature resistant screening medium for future use.

[0026] Adjust the pH value of the growth medium: measure the pH value of the solid growth medium and the liquid growth medium with a pH meter, and adjust the pH value of the periwinkle growth medium, the induction growth medium a...

Embodiment 3

[0032] This embodiment includes the following steps.

[0033] 1) Prepare periwinkle growth medium, induction growth medium and salt-tolerant and low-temperature resistant screening medium respectively: take MS basic medium, add 7 grams of agar powder per liter to obtain periwinkle growth medium for use; take another MS basic medium Culture medium, add 0.2 grams of naphthaleneacetic acid, 3.5 grams of 6-benzylaminopurine, 0.3 grams of colchicine and 7 grams of agar powder per liter as a unit to obtain an induction growth medium for subsequent use; take another MS basic medium, and use it as a unit per liter Add 15 grams of sodium chloride and 7 grams of agar powder as a unit to obtain a salt-tolerant and low-temperature resistant screening medium for future use.

[0034]Adjust the pH value of the growth medium: measure the pH value of the solid growth medium and the liquid growth medium with a pH meter, and adjust the pH value of the periwinkle growth medium, the induction grow...

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PUM

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Abstract

The invention relates to a fast propagation method for cultivating a salt-tolerant and low temperature-tolerant periwinkle strain. The fast propagation method disclosed by the invention is based on preparation of a periwinkle growth culture medium, induction of the growth culture medium and screening of the culture medium in a salt-tolerant and low temperature-tolerant manner, wild periwinkle blades are taken as original materials for inducing an aseptic seedling, and a salt-tolerant and low temperature-tolerant periwinkle strain cultivation system is established by fast propagation and multi-generation induction acclimatization of the aseptic seedling, wherein the alkaloid content of the salt-tolerant and low temperature-tolerant periwinkle strain is higher than that of wild periwinkle by about 20%. The fast propagation method disclosed by the invention has the advantages that, (1) the cultivation process is controllable completely and can not affected by a natural environment; (2) the salt-tolerant and low temperature-tolerant periwinkle can grow normally on a saline-alkali mudflat; and (3) the growth period of the periwinkle in the Yangtze River valley is prolonged and the biomass of the periwinkle is increased.

Description

technical field [0001] The invention relates to a cultivation method of periwinkle, in particular discloses a method for rapid propagation of a salt-tolerant and low-temperature resistant periwinkle strain, and belongs to the technical field of bioengineering. Background technique [0002] Vinca (Catharanthus roseus) is a plant of Apocynaceae, which contains more than 100 kinds of indole alkaloids, most of which have biological activity. The alkaloids extracted or semi-synthesized from Vinca include vinblastine sulfate (vinblastine), vincristine sulfate (vincristine), vindesine sulfate (vinblastine, Vindestine, VDS), vinorelbine tartrate (Vinrelbine, VNB), is At present, the most widely used natural plant anti-tumor drug, there are two vinblastine analogues (anhydrovinblastine anhydrovinblastine and 20,20'-difluorovinorelbine, vinflunine) are under clinical research. The research and development of periwinkle is also one of the most potential and active fields at present. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 倪迪安倪新忠
Owner 上海安体康生植物化学有限公司
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