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Construction method of recombinant HEK (human embryonic kidney) 293 cells highly expressing H1R (histamine receptor 1)

A construction method and high expression technology are applied in the field of recombinant HEK293 cell construction to achieve the effect of improving specificity and sensitivity

Inactive Publication Date: 2012-07-25
XI AN JIAOTONG UNIV
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Problems solved by technology

However, not all "atopy" individuals develop an "anaphylaxis" associated with IgE

Method used

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  • Construction method of recombinant HEK (human embryonic kidney) 293 cells highly expressing H1R (histamine receptor 1)
  • Construction method of recombinant HEK (human embryonic kidney) 293 cells highly expressing H1R (histamine receptor 1)
  • Construction method of recombinant HEK (human embryonic kidney) 293 cells highly expressing H1R (histamine receptor 1)

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Embodiment Construction

[0024] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0025] In the present invention, on the basis of constructing the eukaryotic expression vector pEGFP-N1 / H1R of the full-length H1R gene, HEK293 cells are transfected to obtain a recombinant HEK293 / H1R cell line that is stable and highly expresses H1R. The following is an explanation of the embodiments of the present invention. Not limited.

[0026] In this example, pEGFP-N1 was used as the base vector, and HindIII and XhoI restriction sites were specifically selected as multiple cloning sites for connecting foreign genes.

[0027] A method for constructing recombinant HEK293 cells with high H1R expression, comprising the following steps:

[0028] 1) H1R gene cloning

[0029] Select the pBluescriptR / H1 plasmid containing the full-length cDNA sequence of the H1R gene as a template for cloning, use Primer Premier5.0 software to design corresponding spe...

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Abstract

The invention relates to a construction method of recombinant HEK (human embryonic kidney) 293 cells highly expressing H1R (histamine receptor 1). The construction method comprises constructing an eukaryotic expression vector pEGFP-N1-H1R for a full length H1R gene by utilizing an eukaryotic expression vector pEGFP-N1, transfecting HEK293 cells with the eukaryotic expression vector pEGFP-N1-H1R, and confirming that the cell line can improve the expression of the full length H1R by 2.58 times. The recombinant HEK293-H1R cells highly expressing H1R is applied in screening antiallergic drugs.

Description

technical field [0001] The invention belongs to the technical field of recombinant cells, and relates to a method for constructing recombinant HEK293 cells with high expression of H1R. Background technique [0002] G protein-coupled receptors (GPCRs), also known as seven α-helices transmembrane segment receptors (7TM receptors), is the largest protein superfamily in the body. In terms of structure, it includes seven transmembrane segments. After they bind to the ligand G protein molecular switch, through the mediation of the G protein coupled to the receptor, the second messenger substance is increased or decreased, and in turn changes on the membrane. ion channels that cause changes in membrane potential. Its action is slower than that of ion channel receptors, and the coupling relationship between such receptors and G proteins is quite complicated; one receptor can be coupled with multiple G proteins to activate multiple effector systems; Coupling of several receptors or...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/11C12N15/85C12Q1/02
Inventor 贺浪冲张涛黄静郭瑛
Owner XI AN JIAOTONG UNIV
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