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Application of yeast culture on promoting proliferation of lactic acid-utilizing bacteria

A yeast culture, a technology using bacteria, applied in microorganism-based methods, bacteria, microorganisms, etc., can solve problems such as metabolite composition and concentration differences

Inactive Publication Date: 2012-07-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even when the same yeast strain is used, different medium compositions or different fermentation production control processes can lead to significant differences in the composition and concentration of metabolites in yeast culture products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, yeast culture and its preparation method and application

[0036] 1. Preparation of Yeast Culture

[0037] 1. Preparation of culture medium

[0038] Liquid seed medium: weigh 10g glucose, 5g yeast powder, 5g peptone and 0.1g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0039]Liquid fermentation medium: weigh 10g glucose, 10g sucrose, 10g molasses, 10g peptone, 10g yeast extract, 5g ammonium sulfate, 0.1g KH 2 PO 4 , 0.1g MgSO 4 ·7H 2 O, 0.1g MnSO 4 ·H 2 O, 0.1g CaCl 2 , 0.1gCuSO 4 ·5H 2 O, 0.1g ZnSO 4 ·7H 2 O and 0.1 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 4.0 with 0.1mol / L HCl solution, dilute to 1L, sterilize at 121°C, 0.1Mpa for 15min, and set aside.

[0040] 2. Obtaining of seed solution

[0041] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for thre...

Embodiment 2

[0058] Embodiment 2, yeast culture and its preparation method and application

[0059] 1. Preparation of Yeast Culture

[0060] 1. Preparation of culture medium

[0061] Liquid seed medium: weigh 40g glucose, 40g yeast powder, 40g peptone and 4.0g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0062] Liquid fermentation medium: 100g glucose, 100g sucrose, 100g molasses, 300g peptone, 300g yeast extract, 40g ammonium sulfate, 10g KH 2 PO 4 , 10g MgSO 4 ·7H 2 O, 4.0g MnSO 4 ·H 2 O, 4.0 g CaCl 2 , 4.0g CuSO 4 ·5H 2 O, 4.0g ZnSO 4 ·7H 2 O and 4.0 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 6.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0063] 2. Obtaining of seed solution

[0064] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was act...

Embodiment 3

[0074] Embodiment 3, yeast culture and its preparation method and application

[0075] 1. Preparation of Yeast Culture

[0076] 1. Preparation of culture medium

[0077] Liquid seed medium: 20g glucose, 20g yeast powder, 20g peptone and 2.0g MnSO 4 ·H 2 O.

[0078] Liquid fermentation medium: 50g glucose, 50g sucrose, 50g molasses, 50g peptone, 50g yeast extract, 20g ammonium sulfate, 5.0g KH 2 PO 4 , 5.0 g MgSO 4 ·7H 2 O, 2.0g MnSO 4 ·H 2 O, 2.0g CaCl 2 , 2.0 g CuSO 4 ·5H 2 O, 2.0g ZnSO 4 ·7H 2 O and 2.0 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 5.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0079] 2. Obtaining of seed solution

[0080] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for three generations and then inserted into the above-mentioned liquid seed medium, and cultured with shaking at ...

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Abstract

The invention discloses an application of a yeast culture on promoting proliferation of lactic acid-utilizing bacteria. The yeast culture is prepared by the following steps: liquid fermenting Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882 ), collecting fermentation broth, removing the Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882 ) cells from the fermentation broth to obtain a sterile solution, namely the yeast culture. The experiments show that: the yeast culture is used to respectively culture the Selenomonas maltophilia (Selenomonas ruminantium subsp.ruminantium Bryant ATCC NO.12561) and the Megasphaera elsdenii (Megasphaera elsdenii (Gutierrez et al.) Rogosa ATCC NO.17752) as the lactic acid-utilizing bacteria and the biomasses are respectivelyincreased by 2.6-3.5 times and 2.3-3.8 times. The invention provides an important path for increasing the ruminant productivity level and optimizing the feeder composition.

Description

technical field [0001] The invention relates to the application of a yeast culture in promoting the proliferation of lactic acid utilization bacteria. Background technique [0002] Enhancing the utilization of roughage by ruminants by regulating the structure of rumen microbial flora has become a research hotspot in recent years. Many methods exist to regulate the structure of rumen microflora, such as the application of ionophores and antibiotics. However, the widespread concern about the problems caused by the application of antibiotics in the feed industry and the search for safe feed has prompted researchers to devote themselves to the development of a new type of non-antibiotic or "natural" feed additive. Microbial feed additives, namely direct-fed microorganisms (DFM), fully meet the development needs and have therefore been pushed to the foreground of history. DFM includes bacteria, yeast, etc. Many studies have proved that DFM works well, especially for yeast cultu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12R1/01C12R1/865
Inventor 刘萍解洛香徐乐
Owner CHINA AGRI UNIV
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