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Biological control serratia marcescens strain for general tobacco mosaic virus

A technology for Serratia marcescens and mosaic virus, which is applied in the directions of bacteria, biocides, microorganisms, etc., can solve the problem that the disease prevention mechanism of physiological and biochemical characteristics has not been studied in depth.

Active Publication Date: 2012-07-25
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] There have been reports on the biological control of common tobacco mosaic virus disease in China, but most of them are limited to the screening of bio-control bacteria and pot experiments, and the extraction of antibacterial substances, physiological and biochemical characteristics and disease prevention mechanism of bio-control bacteria, etc. Many aspects have not been studied in depth in China

Method used

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  • Biological control serratia marcescens strain for general tobacco mosaic virus
  • Biological control serratia marcescens strain for general tobacco mosaic virus
  • Biological control serratia marcescens strain for general tobacco mosaic virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1.1 Screening of TMV antagonistic bacteria 2A2 strains

[0029] Pick the purified strain and inoculate it in LB liquid medium, keep the constant temperature at 28°C, and shake at 140rpm for 48 hours, take 20ml and mix it with an equal volume of 40 times TMV juice for 15 minutes, rub the TMV-inoculated rotten spot host Sansheng-NN tobacco, and inoculate the upper part of each strain For 2 leaves, use the mixture of sterile water and TMV as a control, and investigate the dead spot inhibition rate for 3 to 5 days. The strains with antagonistic activity in the preliminary test were tested repeatedly in the same way, with 3 strains for each treatment, and three repetitions.

[0030] The investigation of the rot inhibition rate showed that the strain 2A2 had the best inhibitory effect on TMV, and the TMV rot inhibition rate reached 98%. The biocontrol bacteria produced antibiotic substances with anti-virus activity during the growth and metabolism process. (See Table 1)

[...

Embodiment 2

[0049] 1.1 Practical application of antagonistic bacteria 2A2 in production

[0050] (1) Production of strain 2A2 powder

[0051] 2A2 fermentation broth (1x10 8 cfu / ml) was mixed with white carbon black at a ratio of 6ml:1g, stirred evenly, and dried in a ventilated and lighted place to form a powder. Dilute the dried powder 10X, 50X, 100X, and inoculate it on Sansheng-NN tobacco by the half-leaf method for biological experiments. Treat the diluted powder with the same amount of virus mixture, and use sterile water and the same amount of The virus juice was mixed as a control to detect the effect of inhibiting the virus.

[0052] The inhibitory effect of table 4 dilution powder to virus

[0053]

[0054] After the powder is diluted, it has a good effect of inhibiting the virus. The powder can overcome the difficulty of transportation, is easy to store, and is convenient for production use.

[0055] (2) Comparison of the effects of strain 2A2 with other antiviral agents...

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Abstract

The invention discloses a biological control serratia marcescens strain for a general tobacco mosaic virus. The code name of the strain is 2A2; the strain is preserved in China General Microbiological Culture Collection Center on 7th Sep. 2011; and the preservation number of the strain is CGMCC No.5230. The strain can inhibit the infection of the general tobacco mosaic virus. Biological-control experiments show that the strain can prevent and control the general tobacco mosaic virus. The invention also provides an antagonist polysaccharide obtained from the strain. The antagonist polysaccharide has very high application value in the prevention and control on the general tobacco mosaic virus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Serratia marcescens against tobacco common mosaic virus, the strain and the application of an antagonistic polysaccharide obtained from the strain in the prevention and treatment of tobacco common mosaic virus. Background technique [0002] Tobacco mosaic virus disease is caused by Tobacco mosaic virus (TMV). The most notable symptom is the formation of yellow and green mottled mosaic leaves, and the edges of diseased leaves sometimes curl to the back. The disease can occur continuously throughout the growth period from the seedbed to the field, and the color of the diseased leaves is uneven after modulation, the taste is poor, and the quality declines. Tobacco common mosaic virus is widely distributed in various smoking areas in my country, and Heilongjiang, Jilin, Liaoning, Shandong, Henan, Anhui, Guangdong and other provinces are severely affected. Especially in recent ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12P19/04C12R1/43
Inventor 王凤龙杨金广申莉莉钱玉梅翟熙伦陈德鑫孙惠青王新伟战徊旭
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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