Biological control serratia marcescens strain for general tobacco mosaic virus
A technology of Serratia marcescens and mosaic virus, applied in the direction of bacteria, biocides, microorganisms, etc., can solve the problems of in-depth research on the mechanism of disease prevention of physiological and biochemical characteristics
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Embodiment 1
[0028] 1.1 Screening of TMV antagonistic bacteria 2A2 strains
[0029] Pick the purified strain and inoculate it in LB liquid medium, keep the constant temperature at 28°C, and shake at 140rpm for 48 hours, take 20ml and mix it with an equal volume of 40 times TMV juice for 15 minutes, rub the TMV-inoculated rotten spot host Sansheng-NN tobacco, and inoculate the upper part of each strain For 2 leaves, use the mixture of sterile water and TMV as a control, and investigate the dead spot inhibition rate for 3 to 5 days. The strains with antagonistic activity in the preliminary test were tested repeatedly in the same way, with 3 strains for each treatment, and three repetitions.
[0030] The investigation of the rot inhibition rate showed that the strain 2A2 had the best inhibitory effect on TMV, and the TMV rot inhibition rate reached 98%. The biocontrol bacteria produced antibiotic substances with anti-virus activity during the growth and metabolism process. (See Table 1)
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Embodiment 2
[0049] 1.1 Practical application of antagonistic bacteria 2A2 in production
[0050] (1) Production of strain 2A2 powder
[0051] 2A2 fermentation broth (1x10 8 cfu / ml) was mixed with white carbon black at a ratio of 6ml:1g, stirred evenly, and dried in a ventilated and lighted place to form a powder. Dilute the dried powder 10X, 50X, 100X, and inoculate it on Sansheng-NN tobacco by the half-leaf method for biological experiments. Treat the diluted powder with the same amount of virus mixture, and use sterile water and the same amount of The virus juice was mixed as a control to detect the effect of inhibiting the virus.
[0052] The inhibitory effect of table 4 dilution powder to virus
[0053]
[0054] After the powder is diluted, it has a good effect of inhibiting the virus. The powder can overcome the difficulty of transportation, is easy to store, and is convenient for production use.
[0055] (2) Comparison of the effects of strain 2A2 with other antiviral agents...
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