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WFR (Weil Felix Reaction) test card

A test card and chromatographic membrane technology, applied in the field of immunodetection, can solve the problems of cumbersome operation and long time-consuming, and achieve the effect of short time-consuming and easy operation.

Inactive Publication Date: 2012-07-18
宁波天润生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the existing clinical detection of typhus typhus still using the classic Wai Fiji test existing cumbersome and time-consuming defects, to provide a Wai Fiji test card, which can quickly detect typhus, detection operation Simple and low production cost

Method used

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  • WFR (Weil Felix Reaction) test card
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  • WFR (Weil Felix Reaction) test card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] An outer Fischer test card, comprising a pad 7, the main body of the pad 7 is a plastic pad, the surface of the plastic pad is coated with self-adhesive, and the pad 7 is glued and fixed sequentially from left to right with water-absorbing pads. Performance sample pad 1 (filter paper, Shanghai Gold Standard Biotechnology Co., Ltd.), glass fiber pad 2 (Ahlstrom 8964 glass fiber, Shanghai Jieyi Biotechnology Co., Ltd., model JY-J101), chromatography membrane 5 (nitrocellulose membrane , Millipore Company, model 135s) and absorbent pad 6 with water absorption (filter paper, Shanghai Jinbiao Biotechnology Co., Ltd.), glass fiber pad 2 was evenly sprayed with red latex particles with SPA marks (red polystyrene latex particles, Shanghai Jieyi Biotechnology Co., Ltd., model PSR030N), the method of labeling latex particles is a well-known technology in the art, and will not be repeated here. The right end of the sample pad 1 is lapped on the glass fiber pad 2, and the right end ...

Embodiment 2

[0025] An outer Fischer test card, comprising a pad 7, the main body of the pad 7 is a plastic pad, the surface of the plastic pad is coated with self-adhesive, and the pad 7 is glued and fixed sequentially from left to right with water-absorbing pads. Performance sample pad 1 (filter paper, Shanghai Gold Standard Biotechnology Co., Ltd.), glass fiber pad 2 (Ahlstrom 8964 glass fiber, Shanghai Jieyi Biotechnology Co., Ltd., model JY-J101), chromatography membrane 5 (nitrocellulose membrane , Millipore Co., Ltd., model 180s) and absorbent pad 6 with water absorption (filter paper, Shanghai Jinbiao Biotechnology Co., Ltd.), glass fiber pad 2 is evenly sprayed with red latex particles with SPA mark, and the right end of sample pad 1 is lapped On the glass fiber pad 2, the right end of the glass fiber pad 2 overlaps the left end of the chromatographic membrane 5, the left end of the absorbent pad 6 overlaps the right end of the chromatographic membrane 5, and there is a test line 3...

Embodiment 3

[0028] An outer Fischer test card, comprising a pad 7, the main body of the pad 7 is a plastic pad, the surface of the plastic pad is coated with self-adhesive, and the pad 7 is glued and fixed sequentially from left to right with water-absorbing pads. Performance sample pad 1 (filter paper, Shanghai Gold Standard Biotechnology Co., Ltd.), glass fiber pad 2 (Ahlstrom 8964 glass fiber, Shanghai Jieyi Biotechnology Co., Ltd., model JY-J101), chromatography membrane 5 (nitrocellulose membrane , American Pall Company, model Pall Vivid 170s) and absorbent pad 6 with water absorption (filter paper, Shanghai Jinbiao Biotechnology Co., Ltd.), glass fiber pad 2 was uniformly sprayed with mouse anti-human IgG monoclonal antibody (commercially available, Shanghai Boyao Biotechnology Co., Ltd.) marked red latex particles, the right end of the sample pad 1 is lapped on the glass fiber pad 2, the right end of the glass fiber pad 2 is lapped on the left end of the chromatographic membrane 5, ...

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Abstract

The invention discloses a WFR (Weil Felix Reaction) test card used for detecting typhus rapidly. The WFR test card aims to solve the disadvantage that the classical WFR test stilled adopted for clinical defection of the typhus is fussy to operate and time-consuming. The WFR test card comprises a pad, wherein the pad is provided with a sample pad with water absorbency, a glass fiber strip, a chromatography film and an absorbing pad with water absorbency in a fixing manner sequentially from left to right; the glass fiber strip is uniformly coated with colorful latex particles with marks; the right end of the sample pad is jointed to the glass fiber strip; the right end of the glass fiber strip is jointed to the left end of the chromatography film; the left end of the absorbing pad is jointed to the right end of the chromatography film; the chromatography film is provided with a test line and a control line; the control line is positioned at the right side of the test line; and the test line is formed by fixing one of proteus OX19, proteus OX2 and proteus OXK on the chromatography film. The WFR test card has the advantages of being capable of detecting the typhus rapidly and having simple and convenient detection operations as well as low production cost.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a Fiji test card for rapid detection of typhus. Background technique [0002] At present, the clinical detection of typhus still adopts the classic Waifier's test, which uses the three kinds of diagnostic bacteria in the Waifier's reagent (including Proteus OX19 diagnostic bacteria liquid, Proteus OX2 diagnostic bacteria liquid and Proteus OXK diagnostic bacteria liquid) Test tube agglutination test with the bacterial liquid (bacterial antigen) and the patient’s serum sample to detect the corresponding antibody in the serum, judge the result after 16-18 hours at 37°C, observe the agglutination and precipitation at the bottom of the test tube, if the agglutination titer is ≥ 1:80 For example, the preliminary investigation report on the agglutination test results of 533 healthy people in Datong area (Bao Jifu et al., Shanxi Medical Journal, No. 1, 1978, 42-44) is the above...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543
CPCY02A50/30
Inventor 范亚民李晓霞张捷
Owner 宁波天润生物药业有限公司
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