Preparation method and inspection method for fluorescent magnetic powder dual anti-counterfeiting pelleted seeds
A technology of fluorescent magnetic powder and anti-counterfeiting pills, which is applied in seed coating/seed dressing, chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problem of adding fluorescent anti-counterfeiting technology, and seedlings cannot be used for anti-counterfeiting and detection. problems such as a single method, to achieve the effect of improving the anti-counterfeiting technology system, reducing the possibility of imitation, and protecting economic interests
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Embodiment 1
[0043] Example 1 Preparation of tobacco pelleted seeds
[0044] 10 g of tobacco seeds were poured into the coating machine.
[0045] Granulation: Take 200mg of rhodamine B and add it to distilled water to prepare a rhodamine B aqueous solution with a concentration of 2.0mg / ml. Spray 7.5ml of rhodamine B aqueous solution to moisten the surface of the seeds, add 0.5g of bentonite and keep stirring to make it Wrap it on the surface of the seeds, repeat the above steps 3-5 times to form the seeds into tiny pellets; then add 2-5g of bentonite to the pelleted seeds each time, spray a carboxymethylcellulose solution with a concentration of 1.0%, and keep stirring , repeated several times to increase the particle size of the pelleted seeds to 1.00mm-1.25mm;
[0046] Pelleting: Mix 21g of magnetic powder and 399g of talc powder evenly to obtain a mixture with a magnetic powder content of 5% of the total mass of talc powder and magnetic powder, add 2g to 5g of the mixture of talc pow...
Embodiment 2
[0050] Example 2 Preparation of tobacco pelleted seeds
[0051] Repeat the process of Example 1, the only difference is that in Example 1, 33.6g of magnetic powder and 386.4g of talc powder were uniformly mixed in the pelletizing step to obtain a mixture with a magnetic powder content of 8% of the total mass of talc powder and magnetic powder.
Embodiment 3
[0052] Example 3 Germination of tobacco pelleted seeds
[0053] Prepare a certain amount of contrast pelleted seeds, the specific preparation method is similar to Example 1, the difference is that in the granulation step, rhodamine B solution is sprayed with distilled water, and no magnetic powder is added in the pelletization step to prepare pellets seeds, and the control is represented by CK. The tobacco pelleted seeds prepared in Example 1-2 and the control pelleted seeds were subjected to a germination test on paper, and the seed germination and seedling physiological indexes were measured.
[0054] Wherein the seed germination and seedling physiological index assay methods are as follows:
[0055] (1) Seed germination and seedling indicators
[0056] The pelleted seeds were placed in a 9 cm diameter Petri dish with three layers of absorbent filter paper. Each treatment was replicated 3 times, with 100 pelleted seeds per replicate. The germination experiment was carr...
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