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Preparation method for snakehead virulent ascitesosis disease vaccine

A technology for ascites disease and snakehead, which is applied in the field of preparation of snakehead ascites disease vaccine, can solve the problems of high cost of snakehead ascites, normal colony imbalance, and insignificant effect, and achieves the effects of improving the snakehead immunity, low cost, and no side effects.

Inactive Publication Date: 2012-07-11
李进村
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Problems solved by technology

In addition, due to the feeding of deteriorating bait, the normal bacterial colony in the intestinal tract will be out of balance after the snakehead eats it, creating opportunities for pathogenic bacteria to invade
[0003] In recent years, domestic and imported medicines have no obvious curative effect on snakehead ascites disease or the cost is too high, and the effect is not obvious
It can only be prevented by immunization, and this vaccine is still blank

Method used

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  • Preparation method for snakehead virulent ascitesosis disease vaccine
  • Preparation method for snakehead virulent ascitesosis disease vaccine
  • Preparation method for snakehead virulent ascitesosis disease vaccine

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Embodiment Construction

[0031] The preparation method of snakehead ascites disease vaccine, concrete operation steps are as follows:

[0032] 1. Extract strains: isolate the pathogenic bacterium Citobacillus fischeri from the liver, kidney, blood, and ascites of snakeheads suffering from obvious symptoms of ascites, and immediately store them in a refrigerator at 4-8°C;

[0033] 2. Infection: Infect the isolated pathogenic bacteria with 1-2×10 9 The concentration of each bird is inoculated into healthy snakeheads, and the infection test is carried out indoors to check whether it is virulent, and it is used as a vaccine material if it is proved to be virulent;

[0034] 3. Material collection: take the above-mentioned diseased tissue material of snakehead snakehead, weigh it, add 0.85% of physiological tissue material to each gram of tissue material, and store it in a low-temperature refrigerator at -30°C to -40°C, or put it in 50% phosphate buffered glycerol, and place it for 4-8 days. Store in the r...

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Abstract

The invention provides a preparation method for a snakehead virulent ascitesosis disease vaccine. The method consists of strain extracting, infecting, material preparing, suspension preparing, culture expanding, counting, inactivating, synthesizing, diluting, split charging and detecting. The prepared snakehead virulent ascitesosis disease vaccine is prepared by adding an appropriate amount of double antibodies and preservative into citrobacter freundi. The snakehead virulent ascitesosis disease vaccine has the advantages of low cost, convenience for operating, effective increase in the immunity of snakeheads, prevention of a snakehead virulent ascitesosis disease, easy and convenient use method, safety, nontoxicity, immune protection effect on snakehead cultivation in culturing farms, increase in the cultivation survival rate of snakeheads and good prevention effect on the snakehead virulent ascitesosis disease.

Description

technical field [0001] The invention relates to the field of aquaculture disease prevention and control, in particular to a preparation method of a snakehead ascites disease vaccine. Background technique [0002] Snakeheads belong to Perciformes and Snakeheadidae, commonly known as mullet, blackfish, snakehead, etc. They are rare freshwater fish with high economic value and nutritional value as fish food. The black snakehead has few bone spurs, high meat content, and rich nutrition. According to the measurement, every 100g black snakehead meat contains 19.8g of protein, 1.49g of fat, and 1.2g of carbohydrates, and is rich in calcium, phosphorus, iron, zinc and other nutrients needed by the human body element. Snakehead also has medicinal effects such as removing dampness and treating diseases, eliminating phlegm and promoting regeneration, ventilating and reducing swelling, promoting blood circulation and nourishing, promoting muscle growth and enriching blood. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61P31/04
Inventor 李进村吴多生冯毅章晓红沙正月
Owner 李进村
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