Traditional Chinese medicine health care product, its preparation method and its application
A technology of health care products and traditional Chinese medicine, which is applied in the direction of medical formulas, drug combinations, and medical preparations containing active ingredients, etc., which can solve problems such as production decline, scarce resources, and uncontrollable product quantity and quality, and achieve short growth cycles and improved Anemia, high yield effect
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Embodiment 1
[0051] Embodiment 1 Paecilomyces cicada artificial cultivation
[0052] 1) Inoculate Paecilomyces cicadae strain CGMCC No.3453 on PSA slant medium, and culture at 20°C for 10 days;
[0053] 2) Scrape the mycelium from the slope and inoculate it into the liquid culture medium of the Erlenmeyer flask based on the PDB liquid, at 20°C, 140rpm, cultivate for 3 days to obtain the Paecilomyces cicada shake flask seed liquid; The mold shake bottle seed liquid is 10% by volume and is inserted into the fermenter in which the sterilized PSB liquid is the seed medium. At 20°C, the stirring rate is 140rpm, and the Paecilomyces cicadae fermenter seed can be obtained after cultivating for 5 days. liquid;
[0054] 3) Insert the obtained fermenter seed liquid into the sterilized solid-phase medium at a weight percentage of 5%, and add artificial scattering light sources during solid fermentation at a temperature of 18°C and a humidity of 60%. (Continuously increase the light intensity 150L...
Embodiment 2
[0056] Embodiment 2 Paecilomyces cicada artificial cultivation
[0057] 1) Inoculate Paecilomyces cicadae strain CGMCC No.3453 on PSA slant medium, and culture at 22°C for 9 days;
[0058] 2) Scrape the mycelium from the inclined surface and inoculate it into the liquid culture medium of the Erlenmeyer flask based on the PDB liquid, at 22°C, 140rpm, cultivate for 3 days to obtain the Paecilomyces cicada shake flask seed liquid; The mold shake bottle seed solution is 10% by volume and is inserted into a fermenter in which the sterilized PSB liquid is a seed medium. At 22° C., the stirring speed is 150 rpm, and the Paecilomyces cicadae fermenter seeds can be obtained after culturing for 4 days. liquid;
[0059] 3) Insert the obtained fermenter seed liquid into the sterilized solid-phase culture medium at a weight percentage of 5%, and add an artificial scattering light source when the solid is fermented at a temperature of 21° C. and a humidity of 70%. (Continuously increase t...
Embodiment 3
[0061] Embodiment 3 Paecilomyces cicada artificial cultivation
[0062] 1) inoculate Paecilomyces cicadae strain CGMCC No.3453 on PSA slant medium, and culture at 25°C for 8 days;
[0063] 2) Scrape the mycelia from the inclined surface and inoculate them into the liquid culture medium of the Erlenmeyer flask based on the PDB liquid, at 25°C, 140rpm, and cultivate for 3 days to obtain the Paecilomyces cicada shake flask seed liquid; The mold shake bottle seed liquid is 10% by volume and is inserted into a fermenter in which the sterilized PSB liquid is a seed medium. At 25° C., the stirring rate is 140 rpm, and the Paecilomyces cicadae fermenter seed can be obtained after culturing for 2 days. liquid;
[0064] 3) Insert the obtained fermenter seed liquid into the sterilized solid-phase medium at a weight percentage of 5%, and increase the artificial light source when the solid is fermented under the condition that the temperature is 23° C. and the humidity is 80%. (Continuou...
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