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Rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua

A multi-flowered Polygonatum chinensis and test-tube seedling technology, applied in the field of plant tissue culture, can solve the problems of slow growth rate of seedlings in the later stage, unable to meet the large-scale production of high-quality Polygonatum chinensis seedlings, etc., to shorten the growth cycle, reduce the rate of disinfection and pollution, promote Effects of differentiation and growth

Inactive Publication Date: 2013-04-10
三明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, the existing research is only limited to the use of bud culture, and the growth rate of later seedlings is relatively slow, which cannot meet the large-scale production of high-quality Polygonatum seedlings

Method used

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  • Rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: this Polygonatum polyflora tissue culture rapid propagation method in the present embodiment 1, carries out as follows:

[0032] (1) Preparation of culture medium:

[0033] Medium formula: (I) induction medium MS+1.0mg / L 6-BA+0.5mg / L NAA; (II) proliferation medium MS+1.0mg / L 6-BA+0.5mg / L with buds and tubers NAA+sugar 60g / L; (III) bud part proliferation medium MS+1.0mg / L 6-BA+0.5mg / L 2,4-D+sugar 60g / L; (IV) tuber root part proliferation medium MS +1.0mg / L 6-BA+0.5mg / L NAA+paclobutrazol 0-50mg / L+sugar 60g / L; (V) MS+0.5mg / L 6-BA+0.5mg / L NAA+0.5 % activated carbon; the pH of the above No. I-V mediums are all between 5.6-5.7.

[0034] (3) Root tuber induction culture of Polygonatum multiflora with buds: cut off the surface layer of the tuber with a scalpel on the tuber tuber with buds that have been sterilized, leaving only the main bud plus 0.8-1.2cm 3 The tuber root of access (I) carries out induction culture on No. induction medium, and after 40-50 days,...

Embodiment 2

[0039] Embodiment 2: this Polygonatum polyflora tissue culture rapid propagation method in the present embodiment 2, carries out as follows:

[0040] (1) Preparation of culture medium:

[0041] Medium formula: (I) induction medium MS+1.0mg / L 6-BA+0.5mg / L NAA; (II) proliferation medium MS+1.0mg / L 6-BA+0.5mg / L with buds and tubers NAA+sugar 60g / L; (III) bud part proliferation medium MS+1.0mg / L 6-BA+0.5mg / L 2,4-D+sugar 60g / L; (IV) tuber root part proliferation medium MS +1.0mg / L 6-BA+0.5mg / L NAA+paclobutrazol 0-50mg / L+sugar 60g / L; (V) MS+0.5mg / L 6-BA+0.5mg / L NAA+0.5 % activated carbon; the pH of the above No. I-V mediums are all between 5.6-5.7.

[0042] (2) Selection, pretreatment and disinfection of explants: the materials are selected in September and October of the next year, and the root tubers of Polygonatum polyflora with buds are selected on sunny days, cleaned, and placed in 1 / 700 concentration of methyl Tropone Soak it in cloth for 30 minutes, take it out, place it a...

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Abstract

A rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua relates to the tissue culture technology of plants. The formulas of culture mediums are as follows: an induction culture medium includes MS, 1.0 mg / L of 6-BA, and 0.5 mg / L of NAA, a proliferation culture medium for root tubers with buds include MS, 10 mg / L of 6-BA, 0.5 mg / L of NAA, and 60 g / L of sugar, and a proliferation culture medium for the bud body parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of 2,4-D, and 60 g / L of sugar, a proliferation and seedling strengthening culture medium for the root tuber parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of NAA, 0 to 50 mg / L of paclobutrazol, and 60 g / L of sugar, and a rooting culture medium for the root tuber parts include MS, 0.5 mg / L of 6-BA, 0.5 mg / L of NAA, and 0.5% of activated charcoal. The method comprises the steps as follows: the surface layers of the root tuber parts of the disinfected root tubers with buds are removed, the root tubers with buds are cut into root tubers and buds after being cultivated in the induction culture medium and the proliferation culture medium, the root tubers are transferred to enrichment culture and cultivated circularly for 3 to 6 generations, the buds are transferred to proliferation culture of the bud parts and are proliferated in a compound and circulating manner for 3 to 6 generations, and proliferation strong seedling culture and rooting culture are performed to final end products which are the root tubers with buds. The method is applicable to rapid propagation and scale production.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of high-quality Polygonatum multiflora test-tube seedlings. Background technique: [0002] Polygonatum cyrtonema Hua (Polygonatum cyrtonema Hua) is a medicinal plant of the genus Polygonatum cyrtonema. Its rhizome is used as a traditional Chinese medicine Polygonatum cyrtonema Hua. It contains polysaccharides, amino acids, anthraquinones and other components. It has antibacterial, antihypertensive, anti-aging and treatment of rheumatism. . It is cultivated in some areas in southern my country, and it mainly relies on the asexual reproduction of rhizomes and seeds for reproduction. However, due to the low reproduction coefficient and the slow speed of the seed reproduction cycle, a large number of seedlings cannot be provided, so large-scale planting is limited. In addition, long-term asexual reproduction can easily lead to the degrada...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 林辉锋周辉明王雪凤叶榕妹贺佩珍尚伟
Owner 三明市农业科学研究院
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