Agrobacterium tumefaciens-medicated genetic transformation method of wheat

Abstract

The invention discloses an agrobacterium tumefaciens-medicated genetic transformation method of wheat. The agrobacterium tumefaciens-medicated genetic transformation method comprises the following steps of: firstly, inoculating recombinant Agrobacterium tumefaciens containing a target DNA (Deoxyribonucleic Acid) into wheat immature embryos; and then co-culturing the inoculated wheat immature embryos for enabling the T-DNA of the recombinant Agrobacterium tumefaciens to be integrated with a chromosome of the wheat; and secondly, directly inoculating the wheat immature embryos co-cultured in the first step in a long-seedling culture medium for culturing to obtain transgenic wheat seedlings with roots, stems and leaves. The method disclosed by the invention has the advantages that the applicability is wide, so that the method is suitable for transforming various wheat species; the method is simple in operation, and thus a transgenic plant can be obtained without inducing a tissue culture and regenerating the plant; and the transformation period is short, i.e. the process from the infection to the obtaining of the transgenic wheat seedlings with the roots, the stems and the leaves only requires 11-14 days. The method has very important significance for genetic improvement on the wheat species and the obtaining of new species of the transgenic wheat.

Description

technical field [0001] The invention relates to a wheat genetic transformation method mediated by Agrobacterium. Background technique [0002] Wheat is one of the important cereal crops that human beings depend on for survival. Its planting area and total output exceed other crops in the world. Its genetic improvement has always been an important goal of people's efforts. The traditional wheat breeding technology has limitations such as long breeding cycle and limited yield increase potential. With the development of biotechnology, the use of genetic engineering technology to improve wheat varieties has attracted more and more attention. Compared with other cereal crops such as rice and corn, wheat shows lag and difficulty in the research of plant gene transformation system. Wheat genetic transformation research began in the late 1980s. Since wheat was not a natural host for Agrobacterium and the regeneration of wheat protoplasts was relatively difficult, it was not until 1...

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Problems solved by technology

However, different genotypes of wheat have a great influence on plant regeneration, which further limits the production of transgenic wheat through tissue culture.

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