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Control method for synchronization of menstrual cycles of donor mare and receptor mare

A technology of physiological cycle and control method, applied in the fields of medical science, veterinary instruments, animal delivery, etc., can solve the problems of difficult to ensure the physiological cycle, synchronization, loss of efficacy of mares, etc., to improve the success rate of transplantation and survival rate, technology The process is easy to control and the control target is accurate

Inactive Publication Date: 2012-07-04
QINGDAO DERUI JUNFA BIOLOGY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Equine animals have a long estrus period, generally ranging from 4-7 days and animals with large individual differences. After the simultaneous estrus of horses, it is difficult to ensure concentrated ovulation. menstrual cycle synchronization
In addition, the development of follicles in equine animals is uncontrollable, making it difficult to achieve superovulation
The average number of ovulations per estrous cycle of a mares is 1.2, which makes the general estrus synchronization technology in the prior art ineffective when applied to equine animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Control of recipient mares when their estrus lags behind that of the donor mares. The specific steps of this embodiment include:

[0013] (1) First check the uterus and ovaries of the donor and recipient mares with a B-ultrasound diagnostic instrument, check the mares more than once a day after entering the estrus period, and record the estrus status and follicle growth status of the mares and ovulation time;

[0014] (2) Summarize the B-ultrasound examination results of each mare into a table, so as to track the menstrual cycle of each mare;

[0015] (3) According to the physiological difference between the donor and recipient mares, within the 5th to 14th day of the luteal phase of the mares, choose an opportunity to inject prostaglandin (PG) into the donor or recipient mares. The dosage of PG is 0.2mg / horse / time, the method is intramuscular injection, 1-2 days after the use of PG, the mare can be in estrus; or use PG again the next day; shorten the lutea...

Embodiment 2

[0028] Example 2: Control of the donor mares when their estrus lags behind that of the recipient mares. The specific steps of this embodiment are the same as those in Embodiment 1. The uterus and ovaries of the donor mare C and the recipient mare D are checked every day using an ultrasonic B-ultrasound diagnostic instrument, and the physiological states of C and D are judged and recorded. Use PG or HCG when choosing the timing of the physiological gap; for the convenience of expression, the date when receptor D begins to express estrus is marked as the first day, and its dynamic information is as follows:

[0029] On the first day, the donor C was in the 4th day of the luteal body; the recipient D was in estrus, and the diameter of the follicle was 23mm;

[0030] On the 2nd day, the donor C was in the 5th day of the luteal body; the recipient D was in estrus obviously, and the diameter of the follicle was 28mm. Intramuscular injection of 0.2mgPG to donor C;

[0031] On the t...

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Abstract

The invention belongs to the technical field of embryo transplantation and relates to a control method for synchronization of menstrual cycles of a donor mare and a receptor mare. The control method comprises the steps of respectively examining the donor mare and the receptor mare more than once every day, and recording dynamic information of oestrus states, follicle growth states and ovulation time; making a form for the dynamic information to track, observe and record menstrual cycle information of the maria; using prostaglandin on the donor mare and the receptor mare to enable the maria to be in oestrum according to difference with the dynamic information; measuring the diameters of follicles according to the dynamic information and images after the maria are in oestrum; finally utilizing human chorionic gonadotropin to promote ovulation and generate corpus luteum according to physiological difference; and achieving synchronization of menstrual cycles of the donor mare and the receptor mare. The control method is simple in principle, easily controllable in process, accurate in control, high in efficiency and good in effect.

Description

Technical field: [0001] The invention belongs to the technical field of horse embryo engineering, and relates to a method for controlling the synchronization of the physiological cycles of donor and recipient mares, which is used to make the egg donor and the egg recipient mares be in the same physiological estrous cycle when embryo transplantation is performed on horses. Background technique: [0002] Simultaneous estrus control of animals is a new technology to control estrus in embryo transfer. It refers to the use of hormones and other drugs to break the natural estrus law of female animals and let several female animals appear in estrus at the same time. This method is essential in the process of embryo transfer. Yes, it is necessary to ensure that the physiological cycles of the donor dam and the recipient dam are synchronized in order to facilitate transplantation and embryo implantation. In the embryo transfer process of cattle, sheep and other domestic animals, usua...

Claims

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Application Information

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IPC IPC(8): A61D19/04
Inventor 荣美洁李成修张志鹏
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
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