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Manufacturing method of label-free electrochemical immunosensor for detecting zearalanol

A zearatol, immunosensor technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of excessive organic solvent waste, cumbersome processing process, poor purification effect, etc., to achieve good reproducibility, operation Quick and easy, highly sensitive effects

Inactive Publication Date: 2013-04-17
UNIV OF JINAN
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Problems solved by technology

The pretreatment of these methods utilizes liquid-liquid distribution and conventional SPE column purification and separation, all of which have disadvantages such as cumbersome treatment process, poor purification effect, waste of organic solvents, and long time required to varying degrees.

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  • Manufacturing method of label-free electrochemical immunosensor for detecting zearalanol

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Embodiment 1

[0025] A label-free electrochemical immunosensor for detecting zearalanol and a preparation method thereof, the specific steps of which are as follows:

[0026] (1) Firstly, put the graphene oxide into the quartz tube and pass through argon gas for 10 minutes, then rapidly heat it to 1000°C for 1 minute to obtain graphene, and perform transmission electron microscope characterization on the prepared graphene, as shown in the figure 1, it can be seen that the resulting graphene presents a wrinkled sheet structure, indicating that graphene has been successfully prepared;

[0027] (2) The concentration of graphene synthesized by step (1) is 1.0 mg·mL -1 Graphene solution, additionally prepare a concentration of 1.0mg·mL -1 thionine solution, and 0.5 mL of the obtained graphene solution and thionine solution were mixed for 20 hours, and then ultrasonicated for 10 minutes to obtain a thionine-graphene mixed solution;

[0028] (3) Polish the glassy carbon electrode with a diameter...

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Abstract

The invention discloses a label-free electrochemical immunosensor for detecting zearalanol and a manufacturing method thereof. The manufacturing method of the electrochemical immunosensor comprises the following steps: 1, preparing graphene; 2, preparing a mixed solution from thionine and graphene according to a certain molar ratio; and 3, modifying the electrode surface by the prepared mixed solution through utilizing an electrode surface modification technology, and modifying by an antibody. By connecting the electrochemical immunosensor to an electrochemical workstation, zearalanol in animal derived food extracts can be detected. According to the electrochemical immunosensor of the invention, the specificity is strong, and the sensitivity which is high can reach a pg grade; and only 1-2 minutes are needed to complete one basic detection process. The method is rapid and simple to operate, and the reaction and the result are automatically completed and recorded by an apparatus.

Description

technical field [0001] The invention relates to a label-free electrochemical immunosensor for detecting zearalanol and a preparation method thereof, especially a label-free electrochemical immunosensor for detecting zearalanol developed based on thionine-graphene and its Preparation. Background technique [0002] Zearalenol belongs to the gibberellin class of growth promoters. It was once used as a growth promoter in ruminants and has the effect of promoting protein synthesis. However, due to the weak estrogenic effect of zearalanol, its residue in animal body fluids will cause sexual dysfunction and affect the normal development of secondary sexual characteristics. Under the induction of external conditions, this kind of substance may also cause cancer . At the same time, after the substance is excreted from the animal body, it can also affect human health through drinking water and food. Many countries have banned it from being used in all animal foods, and it cannot be ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/416G01N27/38G01N33/53
Inventor 杜斌魏琴杨健吴丹马洪敏李贺庞雪辉张永芳罗川南李燕闫良国李慧芝代玉雪毛珂霞李玉阳崔振涛冯锐
Owner UNIV OF JINAN
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