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Dyeing method for peripheral nerves of fish larvae and juveniles

A technology of peripheral nerve and staining method, which is applied in the preparation of test samples, can solve the problems of cumbersome staining process and poor staining effect, and achieves the effect of wide application range, convenient long-term storage, and air isolation.

Inactive Publication Date: 2014-02-12
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

There are no domestic reports on the research methods of the distribution of fish peripheral nerves, and foreign related reports mainly focus on the following aspects: hematoxylin nerve staining method, which has poor staining effect; cartilage, bone and nerve triple staining method, the main process is first Alcian blue was used to stain the cartilage blue, followed by alizarin red to stain the hard bone red, and finally the peripheral nerve was stained black with Sudan black, and the staining effect was good, but the staining process was cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Fix the larval Chinese sturgeon specimen (full length 6.2cm) with 10vol% formalin (diluted with 37vol% formaldehyde solution) for 48h, and wash the fixed fish specimen for 12h;

[0026] (2) Put the above fish body into a glass square box, add 0.5wt% KOH solution until the fish body is submerged, and leave it for 1 hour;

[0027] (3) Add 3vol% H dropwise to the above solution 2 O 2 Solution (100ml KOH add 4~6 drops of H 2 O 2 ), put the glass square box under the incandescent lamp overnight to accelerate the fading of the fish epidermal pigment;

[0028] (4) Carry out alcohol dehydration treatment on the fish body, and put them in 30 vol%, 50 vol% and 70 vol% alcohol in sequence for 30 minutes;

[0029] (5) Immerse the fish body in 30wt% Sudan black solution (Sudan black powder is dissolved in 70vol% alcohol to a saturated state; diluted with 70vol% alcohol) for nerve staining, the dyeing time is 8h;

[0030] (6) Carry out alcohol hydration treatment on the fish body, put 70vo...

Embodiment 2

[0033] (1) Fix the fish specimen with 10vol% formalin (diluted with 40vol% formaldehyde solution) for 54h, and rinse the fixed fish specimen for 24h;

[0034] (2) Put the above fish body into plastic, add 0.5wt% KOH solution until the fish body is submerged, and leave it for 2h;

[0035] (3) Add 4vol% H dropwise to the above solution 2 O 2 Solution (100ml KOH add 4~6 drops of H 2 O 2 ), place the plastic overnight under an incandescent lamp to accelerate the fading of fish epidermal pigment;

[0036] (4) Carry out alcohol dehydration treatment on the fish body and put them in 30 vol%, 50 vol%, and 70 vol% alcohol in sequence for 40 minutes;

[0037] (5) Immerse the fish body in a 50wt% Sudan black solution (Sudan black powder is dissolved in 70vol% alcohol to a saturated state; diluted with 70vol% alcohol) for nerve staining in a shaker for 12 hours;

[0038] (6) Carry out alcohol hydration treatment on the fish body, put 70vol%, 60vol%, and 50vol% alcohol in sequence for 2 minutes;

[0...

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PUM

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Abstract

The invention relates to a dyeing method for peripheral nerves of fish larvae and juveniles. The method comprises the following steps of: immersing a fish sample in 0.5 percent by weight of KOH solution and 3-4 percent by volume of H2O2 solution; performing alcohol dehydration processing and then immersing in 30-50 percent by weight of Sudan black solution to perform nerve dyeing; and performing alcohol hydration processing and then placing in 0.5 percent by weight of KOH. The method is easy to operate; the required reagent is less; the dyeing time is greatly shortened; expensive reagents and especial instruments are not required; and the method is easy to operate and stable in result, can be used for researching peripheral nerve distribution of normal fish larvae and juveniles and has a wider application range.

Description

Technical field [0001] The invention belongs to the field of dyeing peripheral nerves of fish, and particularly relates to a method for dyeing peripheral nerves of fish larvae and juveniles. Background technique [0002] The nervous system is very important to the life activities of fish. It is composed of the brain, cranial nerves, spinal cord and spinal nerves. The brain and spinal cord are the central nerves, and the cranial nerves and spinal nerves are the peripheral nerves. The distribution of the peripheral nervous system of fish can be studied for fish. The study of origin, evolution and physiological function provides an important basis. There are no reports on the research method of fish peripheral nerve distribution in China, but related reports abroad mainly focus on the following aspects: hematoxylin nerve staining method, which has poor staining effect; cartilage, bone, nerve triple staining method, the main process is first The cartilage was dyed blue with alcian b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 宋炜马凌波
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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