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Method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans

A technology of Aureobasidium pullulans and pullulan, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low utilization rate of raw materials, high cost, high energy consumption, etc., and achieve energy saving, The effect of reducing costs and improving raw material utilization

Active Publication Date: 2012-06-13
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, what the existing technology does is only for the fermentation of polymalic acid or pullulan, which has problems such as low utilization rate of raw materials, high energy consumption, and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Use 30L general-purpose fermenter to configure 18L fermentation medium, medium formula: sucrose 80g / L, peptone 5g / L, calcium carbonate 5g / L, potassium chloride 0.1g / L, sodium nitrate 1g / L, magnesium sulfate 0.1g / L, potassium dihydrogen phosphate 0.05g / L, Mn 2+ , Fe 2+ , Zn 2+ Each at 0.001g / L-0.4g / L, sterilized at 121°C for 20 minutes. After the temperature of the culture medium drops to 28°C, insert the strains, insert the inoculum into the fermenter at an inoculum volume of 3% (the volume ratio of the seed liquid to the fermentation liquid), the stirring speed is at 200rpm, the ventilation ratio is 0.5VVM, and the temperature is controlled at 28°C Start fermentation around 28°C-31°C from the start of fermentation to 72 hours, add sucrose from 24 hours, control the glucose content in the fermentation broth at 35g / LL, control the temperature at about 25°C after 72 hours, and continue Add sucrose to control the glucose content in the fermentation broth to 15g / L, stop...

Embodiment 2

[0022] Use 30L general-purpose fermenter to configure 18L fermentation medium, medium formula: sucrose 135g / L, peptone 30g / L, calcium carbonate 30g / L, potassium chloride 1g / L, sodium nitrate 2g / L, magnesium sulfate 0.4g / L L, potassium dihydrogen phosphate 2.8g / L, Mn 2+ , Fe 2+ , Zn 2+ Each at 0.25g / L, according to the conventional sterilization method, sterilize the solid tank at 121°C for 20min, and insert the strains after the temperature of the medium drops to 28°C, and use 6% (volume ratio of seed liquid to fermentation liquid) The inoculation amount is connected to the fermenter, the stirring speed is 400rpm, the ventilation ratio is 1VVM, and the fermentation is started at a controlled temperature of about 28°C. From the beginning of fermentation to 72 hours, the temperature is controlled at 28°C-31°C, and sucrose is added from 24 hours to control fermentation. The amount of glucose in the liquid was 40g / L, and after 72 hours, the temperature was controlled at about 25...

Embodiment 3

[0024]Use 30L general-purpose fermenter to configure 18L fermentation medium, medium formula: sucrose 200g / L, peptone 50g / L, calcium carbonate 40g / L, potassium chloride 2g / L, sodium nitrate 3g / L, magnesium sulfate 0.6g / L L, potassium dihydrogen phosphate 4g / L, Mn 2+ , Fe 2+ , Zn 2+ Each at 0.4g / L, according to the conventional sterilization method, sterilize the solid tank at 121°C for 20min, and insert the strains after the temperature of the medium drops to 28°C, according to the volume ratio of 10% (the volume ratio of seed liquid to fermentation liquid) The inoculum is connected to the fermenter, the stirring speed is 600rpm, the ventilation ratio is 1.5VVM, the fermentation is started at a controlled temperature of about 28°C, the temperature is controlled at 28°C-31°C from the beginning of fermentation to 72 hours, and sucrose is added from 24 hours to The amount of glucose in the fermentation broth is 50g / L. After 72 hours, control the temperature at about 25°C, and c...

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PUM

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Abstract

The invention discloses a method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans. The invention relates to a method for producing poly(malic acid) and pullulan by using Aureobasidium pullulans, belonging to the technical field of the bio-fermentation engineering. The method comprises the following steps: reducing the temperature of the culture medium of a fermentation tank to 28 DEG C, inoculating a strain in the fermentation tank according to the inoculation quantity which is 3%, stirring under the conditions that the rotational speed is 200rpm and the ventilation rate is 0.5VVM, controlling the temperature to 28-31 DEG C to begin fermentation, performing the fed-batch of sucrose after 24 hours to control the content of sucrose in the fermentation liquor to 35g / L, controlling the temperature to about 25 DEG C after 72 hours, continuously performing the fed-batch of sucrose to control the content of sucrose in the fermentation liquor to 15 g / L, fermenting for 136 hours to stop adding sucrose, continuously fermenting for 8 hours when the content of sucrose in the fermentation liquor is reduced to 0, controlling the pH value to 5-7.2 in the fermentation process, and fermenting for 166 hours. By adopting the method disclosed by the invention, the raw material utilization rate can be increased, energy can be saved and the cost can be reduced.

Description

technical field [0001] The invention relates to a method for producing polymalic acid and pullulan through cells of Aureobasidium pullulans, belonging to the technical field of biological fermentation engineering. Background technique [0002] Both polymalic acid and pullulan are products of Aureobasidium pullulans and can be produced by Aureobasidium pullulans fermentation. In order to increase the yield of polymalic acid or pullulan, people have constructed strains with strong synthetic ability through mutagenesis or genetic engineering, screened and optimized the medium formula, established and optimized the fermentation process control, and improved and enhanced the downstream engineering. Technology and other aspects to improve the yield and quality of products. [0003] Polymalic acid is a high molecular polymer polymerized from malic acid as the only monomer. At present, there are very few patents on microbial fermentation of polymalic acid in foreign countries. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12P19/04C12R1/645
Inventor 乔长晟郝华璇敖爱华盖丽丰姜少丽
Owner 天津北洋百川生物技术有限公司
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