Tissue engineering conduction bundle and construction method and application thereof
A technology for tissue engineering and construction methods, applied in the field of biomedical engineering
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Embodiment 1
[0028] Example 1: Induced canine cardiac conduction cells were implanted into collagen sponges to construct tissue-engineered conduction bundles
[0029] 1. Preparation of cardiac conduction cells by inducing canine bone marrow mesenchymal stem cells
[0030] (1) Isolation and culture of canine bone marrow mesenchymal stem cells and canine atrial appendage myocytes
[0031] A. Isolation and culture of bone marrow mesenchymal stem cells
[0032] Bone marrow mesenchymal stem cells cultured by direct adherence method of canine whole bone marrow: extract 10ml of iliac bone marrow from adult hybrid dogs (Experimental Animal Center, Second Military Medical University, 15kg), centrifuge at 900×g for 10min, discard supernatant and fat; add red blood cells to lyse Liquid TrisNH 4 Cl, remove red blood cells at 4°C for 5 min, add 1×10 6 / ml was inoculated in a culture dish coated with fibronectin (FN) (Sigma Company), and the culture medium was 60% DMEM-F12 (Gibco Company), 10% FCS (G...
Embodiment 2
[0053] Example 2: The rest are the same as in Example 1, and the concentration of paracrine factors endothelin-1 and neuregulin-1 is selected to be 1.5×10 in the induction of bone marrow mesenchymal stem cells to the direction of cardiac conduction cells. -8 M, the induction culture time is selected as 6 days, and the cell planting density is selected as 4×10 in the tissue engineering conduction bundle construction. 5 piece / cm 3 .
Embodiment 3
[0054] Example 3: The rest is the same as in Example 1, and the bone marrow mesenchymal stem cells and atrial appendage muscle cells are selected from rats.
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Application Information
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