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Method for extracting apramycin

A technology of apramycin and tobramycin, which is applied in the field of medicine to achieve the effects of improving utilization, reducing production costs, and reducing environmental risks

Inactive Publication Date: 2012-05-30
PEKING UNIV FOUNDER GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] But so far, there is no documented method for extracting apramycin from the by-product of tobramycin production

Method used

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  • Method for extracting apramycin

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Effect test

Embodiment 1

[0044] 18000L of carbamoyl tobramycin fermentation broth with carbamoyl tobramycin content of 2550μ / ml was adjusted to pH 5.5 with 5mol / L HCl solution, then 700L of 732 resin was added, stirred and adsorbed for 8 hours, and then the resin was collected. And move it to a resin column, use ammonia water with a concentration of 2 mol / L as the eluent for elution, collect 2100 L of eluate, and the content of carbamoyl tobramycin is 18000 μ / ml.

[0045] Adjust the concentration of carbamoyl tobramycin in the eluate to 25450 μ / ml, heat to 80-90° C., and hydrolyze for 2 hours.

[0046] Then use the HCl solution with a concentration of 2mol / L to adjust the pH of the hydrolyzed solution to 8.5, and then add it to the D151 resin column for adsorption. Carry out elution for the ammoniacal liquor of 0.5mol / L, the flow rate of controlling ammoniacal liquor is 2000ml / min.

[0047] During the elution with ammonia water, the components of the eluate were monitored by TLC thin layer chromatogr...

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Abstract

The invention provides a method for extracting apramycin, comprising the following steps: purifying a Streptomyces tenebrarius broth for producing carbamoyltobramycin through a cation resin to obtain a rough solution of carbamoyltobramycin containing apramycin; carrying out hydrolysis to obtain a rough solution of tobramycin containing apramycin; and then purifying through the apramycin to obtain an apramycin solution. According to the method, a by-product apramycin can be extracted while apramycin is produced from the Streptomyces tenebrarius broth, thus utilization rate of the by-product in the production process of tobramycin is raised, and simultaneously the production cost of the product is reduced, and the environmental protection risk is reduced.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a preparation method of a veterinary crude drug, in particular to a method for extracting and refining apramycin from carbamoyl tobramycin fermentation liquid. Background technique [0002] Apramycin (Apramycin) has another name called apramycin, belongs to aminocyclic alcohol compounds, and its structure is as shown in formula (I): [0003] [0004] Apramycin was successfully developed by the United States in the 1980s. Using apramycin as a drug-type feed additive can obviously promote animal weight gain and improve feed conversion rate, so it is widely used in the field of livestock and poultry breeding. Apramycin is characterized by a broad antibacterial spectrum and is not prone to drug resistance. It is effective against Gram-positive bacteria, some Gram-negative bacteria, and also effective against some mycoplasma. The most sensitive to apramycin are Escherichia coli, Salmonella...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/22C07H1/06B01D15/04
Inventor 徐红蔡丽赵德
Owner PEKING UNIV FOUNDER GRP CO LTD
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